USE OF RADIOLABELED MONOCLONAL ANTI-CEA ANTIBODIES FOR THE DETECTION OF HUMAN CARCINOMAS BY EXTERNAL PHOTOSCANNING AND TOMOSCINTIGRAPHY
USE OF RADIOLABELED MONOCLONAL ANTI-CEA ANTIBODIES FOR THE DETECTION OF HUMAN CARCINOMAS BY EXTERNAL PHOTOSCANNING AND TOMOSCINTIGRAPHY
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DOI：
10.1016/0167-5699(81)90011-6
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
CARREL, S
中科院分区：
文献类型：
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作者：
MACH, JP;BUCHEGGER, F;CARREL, S
保罗埃尔利希的灵感概念“神奇子弹”的治愈(~f疾病已经重振了~最近的进步免疫学j。细胞融合技术的发展，使生产r！[单抗(单抗)与精致的特异性2激发了新的希望，我们现在可能有完美的载体分子，以提供细胞毒药物3或毒素4隐藏的癌细胞。这篇文章回顾了神奇子弹概念的一个方面的数据，即使用放射性标记抗体作为道岔定位的示踪剂。本文还将讨论13I标记的抗癌胚抗原(CEA)单抗在临床上的最新应用，无论是常规的体外光敏扫描还是单光子发射增强扫描(SPECT)。这种联合(来自免疫学(MAb)和核医学(SPECT)的最现代工具)似乎是一种很有希望的方法，可以提高‘免疫核素照相’的灵敏度。然而，这种方法还没有被广泛应用于临床。放射性标记抗体的肿瘤定位的实验模型研究是由Pressman C‘和Bale 7在近30年前开始的，他们表明针对Wagner骨肉瘤或Walker癌细胞的标记抗体在体内是浓缩的。普雷斯曼在这一领域的主要贡献之一是引入了配对标记方法8，在这种方法中，抗体和对照正常免疫球蛋白同时被注射到同一只有投票率的动物中，每种抗体都标记了不同的同位素。通过测量双通道闪烁计数器中每个同位素的放射性，可以区分抗体在正常免疫球蛋白的非特异性聚集中的特定定位，这是已知发生在肿瘤的炎症和坏死区的。1971年，使用这个
Paul Ehrlich's inspired concept of'magic bullets' for the cure (~ f diseases has beer~ revitalized by recent advances in immunology j. In parlicular, the development of ceil fusion technology allowing the production r![monoelonal antibodies (Mabs) with exquisite specifcities 2 triggered new hopes that we may now have the perfect carrier molecules with which to deliver cytotoxic drugs 3 or loxins 4 to the hidden cancer cells. This article reviews data on one aspect of the magic bullet concept, the use of radiolabelled antibodies as tracers for turnout localization. It will also discuss the very recent clinical use of 13l I-labelled Mabs against carcinoembryonic antigen (CEA)~ lo detect carcinoma either by conventional external photosearmir~ g or by single photon emission compulerized lomography (SPECT). This alliance (the most modern tools from immunology (Mabs) and nuclear medicine (SPECT) appears promising as a way to improve the sensitivity of'immunoscintigraphy'. However, this approach is not yet tea+ for wi&spread clinical use.Experimental models Research on tumour localization of radiolabelled antibodies was initiated almost 30 years ago by Pressman C'and Bale 7, who showed that labelled antibodies against Wagner osteosarcoma or Walker carcinoma cells were concentrated in vivo by these turnouts. One of Pressman's major contributions in this field was to introduce the paired labelling method 8, in which both antibodies and control normal IgG, each labelled with a different isotope, are injected simultaneously into the same turnout-bearing animal. The measurement of radioactivity from each isotope in a dual channel scintillation counter allows one to distinguish the specific localization of antibodies in a turnout ft'om the non-specific accumulation of normal IgG, which is known to occur in the inflammatory and necrotic region of the tumour'. In 1971, using this