Identification and functional importance of IL-1 receptors on rat parietal cells
Identification and functional importance of IL-1 receptors on rat parietal cells
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DOI：
10.1152/ajpgi.1998.275.5.g1094
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发表时间：
1998-11-01
影响因子：
4.5
通讯作者：
Prinz, C
中科院分区：
文献类型：
--
作者：
Schepp, W;Dehne, K;Prinz, C
我们研究了白细胞介素-1（IL-1）受体的表达和IL-1 β对高度富集（>97%）大鼠壁细胞功能的影响。使用大鼠IL-1受体特异性引物对壁细胞poly（A）（+）RNA进行RT-PCR，发现单个547-kb PCR产物与已发表的IL-1受体序列高度同源。北方印迹分析表明，大鼠壁细胞和脑的poly（A）（+）RNA为5.7kb的单一RNA。用生物素化重组人IL-1 β进行壁细胞IL-1受体的细胞化学，通过抗生物素蛋白偶联荧光素显色。对应于高度的壁细胞富集，95%的细胞染色阳性。基础H+产生（[C-14]氨基比林积累）不受IL-1 β（0.25-100 pg/ml）的影响，当与细胞因子同时加入时，对组胺或卡巴胆碱的反应也不受影响。然而，当壁细胞在加入组胺或卡巴胆碱之前与IL-1 β（0.5-5 pg/ml）预孵育10分钟时，对这些促分泌素的反应分别降低了35%和67%。人重组IL-1受体拮抗剂可完全逆转IL-1 β的抑制作用。壁细胞与IL-1 β预孵育未能改变组胺刺激的cAMP产生，但显着抑制卡巴胆碱诱导的D-肌肌醇1，4，5-三磷酸形成。在fura 2加载，纯化的壁细胞，10分钟的预孵育与IL-1 β显着降低初始瞬时峰值升高细胞内钙离子浓度响应卡巴胆碱。我们的结论是，大鼠壁细胞表达IL-1受体介导抑制H+生产。IL-1 β的抗分泌作用可能导致继发于急性幽门螺杆菌感染或幽门螺杆菌慢性定植过程中的胃酸过少。幽门偏爱胃底粘膜。
We studied the expression of interleukin-1 (IL-1) receptors and the effect of IL-1 beta on the function of highly enriched (>97%) rat parietal cells. RT-PCR of parietal cell poly(A)(+) RNA with primers specific for the rat IL-1 receptor revealed a single 547-kb PCR product highly homologous to the published sequence of the IL-I receptor. Northern blot analysis of poly(A)(+) RNA of rat parietal cells and brain revealed a single RNA species of 5.7 kb. Cytochemistry of parietal cell IL-1 receptor was performed with biotinylated recombinant human IL-1 beta, visualized by avidin-coupled fluorescein. Corresponding to the high degree of parietal cell enrichment, 95% of the cells stained positive. Basal H+ production ([C-14]aminopyrine accumulation) was not changed by IL-1 beta (0.25-100 pg/ml) nor was the response to histamine or carbachol when added simultaneously with the cytokine. However, when parietal cells were preincubated with IL-1 beta (0.5-5 pg/ml) for 10 min before the addition of histamine or carbachol, the response to these secretagogues was reduced by 35 and 67%, respectively. Inhibition by IL-1 beta was fully reversed by the human recombinant IL-1 receptor antagonist. Preincubation of parietal cells with IL-1 beta failed to alter histamine-stimulated cAMP production but markedly inhibited carbachol-induced formation of D-myo-inositol 1,4,5-trisphosphate. In fura 2-loaded, purified parietal cells, 10 min preincubation with IL-1 beta dramatically reduced the initial transient peak elevation of intracellular Ca2+ concentration in response to carbachol. We conclude that rat parietal cells express IL-I receptors mediating inhibition of H+ production. The antisecretory effect of IL-1 beta may contribute to hypoacidity secondary to acute Helicobacter pylori infection or during chronic colonization by H. pylori preferring the fundic mucosa.