(E)-2-hexenal fumigation control the gray mold on fruits via consuming glutathione of Botrytis cinerea.
(E)-2-hexenal fumigation control the gray mold on fruits via consuming glutathione of Botrytis cinerea.
复制标题
DOI：
10.1016/j.foodchem.2023.137146
复制
发表时间：
2023-08
期刊：
影响因子：
8.8
通讯作者：
Xiaocheng Zhang;Dong Li;Z. Luo;Yanqun Xu
中科院分区：
文献类型：
--
作者：
Xiaocheng Zhang;Dong Li;Z. Luo;Yanqun Xu
（E）-2-己烯醛熏蒸抑制了葡萄孢菌的生长。灰霉病的直接作用靶点及其对采后草莓的影响尚未发现。在本研究中，我们将（E）-2-己烯醛熏蒸水平从0.524 μM增加到1048 μM。草莓和中等大小。结果表明，随着剂量的增加，（E）-2-己烯醛熏蒸对草莓病斑直径的抑制率分别为13.96%、20.41%和100%。在培养液中，（E）-2-己烯醛熏气处理能增加B.灰叶LC-MS/MS和FTIR结果证明（E）-2-己烯醛和谷胱甘肽之间发生1：1迈克尔加成反应，产物为GSH-H inB。用（E）-2-己烯醛熏蒸。此外，B. L.在熏蒸过程中，谷胱甘肽和谷胱甘肽二硫化物的消耗与GSH-H的产生沿着。（E）-2-己烯醛引起的灰化反应具有浓度和时间依赖性。本研究定位了（E）-2-己烯醛对B的直接作用靶点，发现了（E）-2-己烯醛对B的功能模型。灰叶
(E)-2-hexenal fumigation inhibits the growth ofBotrytis. cinerea, but the direct target and its effect on postharvest strawberry have not yet been discovered. In the present study, we applied increasing level of (E)-2-hexenal fumigation from 0.524 μM to 1048 μM toB. cinereaon strawberry and medium. Results showed that (E)-2-hexenal fumigation inhibited lesion diameter on strawberry by 13.96 %, 20.41 % and 100 % with the dosage increasing. On medium, (E)-2-hexenal fumigation increased both the ROS and mitochondria membrane potential level ofB. cinerea. LC-MS/MS and FTIR results demonstrate a 1:1 Michael addition reaction between (E)-2-hexenal and glutathione with the product GSH-H inB. cinereaunder (E)-2-hexenal fumigation. Furthermore, the consumption of glutathione and glutathione disulfide along with the production of GSH-H during fumigation inB. cinereacaused by (E)-2-hexenal were both concentration- and time-dependent. This study locates the direct target and discovered the functional model of (E)-2-hexenal toB. cinerea.