Characterization and chromosomal mapping of the human TFG gene involved in thyroid carcinoma
Characterization and chromosomal mapping of the human TFG gene involved in thyroid carcinoma
复制标题
DOI：
10.1006/geno.1997.4625
复制
发表时间：
1997-05-01
期刊：
影响因子：
4.4
通讯作者：
Aman, P
中科院分区：
文献类型：
--
作者：
Mencinger, M;Panagopoulos, I;Aman, P
使用SPYGQ富集区作为查询序列在表达序列标签数据库中进行同源性搜索，以找到编码与肉瘤相关EWS和FUS蛋白的N-末端部分相似的蛋白质区域的基因。选择克隆22911（T74973），其在其5'端编码SPYGQ富集区，以及与22911重叠的几个其它克隆。该cDNA全长1677 bp，含有一个起始密码子ATG，一个400个氨基酸的开放阅读框，一个poly（A）信号，加上一个poly（A）尾。我们发现在共有序列的5'部分和命名为TFG的598 bp长的序列之间100%相同。TFG序列融合到NTRK 1的3'末端，产生在乳头状甲状腺癌中发现的TRK-T3融合转录物。因此，cDNA代表TFG基因的全长转录物。荧光原位杂交显示TFG定位于3q 11-q12。TFG cDNA探针的3'端和5'端在所检查的所有组织中与北方印迹过滤器上的2.2-kb条带杂交。(C)北京：科学出版社.
Homology searches in the Expressed Sequence Tag Database were performed using SPYGQ-rich regions as query sequences to find genes encoding protein regions similar to the N-terminal parts of the sarcoma-associated EWS and FUS proteins. Clone 22911 (T74973), encoding a SPYGQ-rich region in its 5' end, and several other clones that overlapped 22911 were selected. The combined data made it possible to assemble a full-length cDNA sequence, This cDNA sequence is 1677 bp, containing an initiation codon ATG, an open reading frame of 400 amino acids, a poly(A) signal, add a poly(A) tail. We found 100% identity between the 5' part of the consensus sequence and the 598-bp-long sequence named TFG. The TFG sequence is fused to the 3' end of NTRK1, generating the TRK-T3 fusion transcript found in papillary thyroid carcinoma. The cDNA therefore represents the full-length transcript of the TFG gene. TFG was localized to 3q11-q12 by fluorescence in situ hybridization. The 3' and the 5' ends of the TFG cDNA probe hybridized to a 2.2-kb band on Northern blot filters in all tissues examined. (C) 1997 Academic Press.