Biomarkers of One-Carbon Metabolism Are Associated with Biomarkers of Inflammation in Women
Biomarkers of One-Carbon Metabolism Are Associated with Biomarkers of Inflammation in Women
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DOI：
10.3945/jn.113.183970
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发表时间：
2014-05-01
影响因子：
4.2
通讯作者：
Ulrich, Cornelia M.
中科院分区：
文献类型：
--
作者：
Abbenhardt, Clare;Miller, Joshua W.;Ulrich, Cornelia M.
叶酸介导的一碳代谢对DNA合成、修复和甲基化至关重要。一碳代谢的紊乱与某些癌症的风险增加有关，也可能影响炎症过程。我们研究了这些相互关联的途径，以了解它们之间的关系。目的是探索炎症与营养状况和一碳代谢的生物标志物之间的关联。在从妇女健康倡议观察性研究中选择的1976名妇女的横断面研究中，测量血浆维生素9-6 [吡哆醛-5 '-磷酸（PLP）]、血浆维生素B-12、血浆叶酸和RBC叶酸作为营养生物标志物;测量血清C-反应蛋白（CRP）和血清淀粉样蛋白A（SAA）作为炎症生物标志物;同型半胱氨酸和半胱氨酸被测量为一碳代谢的综合生物标志物。学生的t，卡方，和斯皮尔曼等级相关性，沿着与多元线性回归，被用来探索生物标志物之间的关系，此外，我们测试了分层叶酸强化期和多种维生素的使用。使用单变量分析，血浆PLP是唯一与血清CRP和SAA中度显著相关的营养生物标志物（rho分别= -0.22和-0.12; P < 0.0001）。同型半胱氨酸（μ mol/L）与所有营养生物标志物呈显著负相关（范围从rho = -0.30到rho = -0.46;所有P < 0.0001）。采用多元线性回归分析，血浆PLP、RBC叶酸、同型半胱氨酸和半胱氨酸被确定为CRP的独立预测因子; PLP、维生素B-12、RBC叶酸和同型半胱氨酸被确定为SAA的预测因子。当按叶酸强化期分层时，营养-同型半胱氨酸相关性在强化后普遍较弱，而血浆PLP和血清CRP之间的相关性增加。炎症的生物标志物与女性中的PLP、RBC叶酸和同型半胱氨酸相关。这些途径之间的联系需要进一步调查，因果关系需要建立。该试验在clinicaltrials.gov上注册为NCT 00000611。
Folate-mediated one-carbon metabolism is essential for DNA synthesis, repair, and methylation. Perturbations in one-carbon metabolism have been implicated in increased risk of some cancers and may also affect inflammatory processes. We investigated these interrelated pathways to understand their relation. The objective was to explore associations between inflammation and biomarkers of nutritional status and one-carbon metabolism. In a cross-sectional study in 1976 women selected from the Women's Health Initiative Observational Study, plasma vitamin 9-6 [pyridoxal-5'-phosphate (PLP)], plasma vitamin B-12, plasma folate, and RBC folate were measured as nutritional biomarkers; serum C-reactive protein (CRP) and serum amyloid A (SAA) were measured as biomarkers of inflammation; and homocysteine and cysteine were measured as integrated biomarkers of one-carbon metabolism. Student's t, chi-square, and Spearman rank correlations, along with multiple linear regressions, were used to explore relations between biomarkers; additionally, we tested stratification by folic acid fortification period and multivitamin use. With the use of univariate analysis, plasma PLP was the only nutritional biomarker that was modestly significantly correlated with serum CRP and SAA (rho = -0.22 and -0.12, respectively; P < 0.0001). Homocysteine (mu mol/L) showed significant inverse correlations with all nutritional biomarkers (ranging from rho = -0.30 to rho = -0.46; all P < 0.0001). With the use of multiple linear regression, plasma PLP, RBC folate, homocysteine, and cysteine were identified as independent predictors of CRP; and PLP, vitamin B-12, RBC folate, and homocysteine were identified as predictors of SAA. When stratified by folic acid fortification period, nutrition-homocysteine correlations were generally weaker in the postfortification period, whereas associations between plasma PLP and serum CRP increased. Biomarkers of inflammation are associated with PLP, RBC folate, and homocysteine in women. The connection between the pathways needs to be further investigated and causality established. The trial is registered at clinicaltrials.gov as NCT00000611.