TEMPLATE-DIRECTED OLIGONUCLEOTIDE LIGATION ON HYDROXYLAPATITE
TEMPLATE-DIRECTED OLIGONUCLEOTIDE LIGATION ON HYDROXYLAPATITE
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DOI：
10.1038/321790a0
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发表时间：
1986-06-19
期刊：
影响因子：
64.8
通讯作者：
ORGEL, LE
中科院分区：
文献类型：
--
作者：
ACEVEDO, OL;ORGEL, LE
伯纳尔1和随后的其他作者2提出，生物聚合物前体的生前合成可能发生在固体表面，如粘土或某些其他矿物提供的表面。在这样的体系中，从反应混合物的其他成分中分离产物使人想起现代多肽和多核苷酸的固相合成。一种这样的方案设想，生长的聚合物通过吸附到矿物表面来局部化，在那里活化剂或活化的单体连续或循环供应3，4。我们一直试图使用我们认为可能与益生菌进化过程中发生的反应有关的反应来测试这一方案。我们已经报道，吸附在羟基磷灰石上的寡核苷酸为(鸟苷5‘-磷)-2-甲基咪唑(2-MeImpG)4-7的齐聚提供了合适的模板。然而，这不是一个合适的测试反应，因为2-MeImpG齐聚几乎一步完成。在本文中，我们报道了在聚(C)的存在下，最初形成的寡聚(G)S通过转化为磷咪唑类化合物而被重新激活，然后允许连接的一系列反应是理想的，因为可以在羟基磷灰石表面进行重复循环，而在液体中实现循环是相当困难的。
Bernal1, and subsequently other authors2, have suggested that the prebiotic synthesis of the precursors of biopolymers could have occurred on a solid surface such as that provided by clay or some other mineral. The separation of products from the other components of the reaction mixture in such a system is reminiscent of modern solid-phase synthesis of polypeptides and polynucleotides. One such scheme envisages that growing polymers were localized by adsorption to a mineral surface where an activating agent or activated monomers were supplied continuously or cyclically3,4. We have been trying to test this scheme using reactions which we believe may be related to those that occurred during prebiotic evolution. We have already reported that oligonucleotides adsorbed onto hydroxylapatite provide suitable templates for the oligomerization of (guanosine 5′-phosphor)-2-methylimidazolide (2-MeImpG)4–7. However, this is not a suitable test reaction, as 2-MeImpG oligomerization proceeds almost to completion in a single step. Here we report that a sequence of reactions in which initially formed oligo(G)s are reactivated by conversion to phosphorimidazolides in the presence of poly(C) and then allowed to ligate is ideal, in that repeated cycles can be carried out on the surface of hydroxylapatite, whereas in the liquid phase the cycle could be achieved only with considerable difficulty.