A sequence-ready 840-kb PAC contig spanning the candidate tumor suppressor locus DBC1 on human chromosome 9q32-q33
A sequence-ready 840-kb PAC contig spanning the candidate tumor suppressor locus DBC1 on human chromosome 9q32-q33
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DOI：
10.1006/geno.1999.5891
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发表时间：
1999-08-01
期刊：
影响因子：
4.4
通讯作者：
Knowles, MA
中科院分区：
文献类型：
--
作者：
Nishiyama, H;Hornigold, N;Knowles, MA
膀胱癌的一个假定的肿瘤抑制基因位点已定位于人类染色体9 q32-q33，并命名为DBC 1。我们先前的微卫星缺失定位研究表明，DBC 1定位在D9 S1848和AFMA 239 XA 9之间。我们已经构建了一个840 kb的序列准备重叠群组成的噬菌体P1衍生的人工染色体（PAC），其中包括DBC 1。克隆最初通过筛选具有通过物理作图定位于该区域的标记的PAC文库来鉴定，随后使用PAC末端探针来完成重叠群。该重叠群包含D9 S1848和AFMA 239 XA 9之间的6个PAC克隆的最小平铺路径。通过筛选辐射杂交定位到周围区域的24个EST，将3个表达序列标签（EST）定位到DBC 1区域。其中一个代表DBCCR 1的基因，这是一个已知的DBC 1候选基因，另外两个是新的。该重叠群和初步表达图谱构成了在该区域鉴定膀胱癌肿瘤抑制基因的基础。(C)北京：科学出版社.
A putative tumor suppressor locus involved in bladder cancer has been mapped to human chromosome 9q32-q33 and designated DBC1. Our previous microsatellite-based deletion mapping study indicated that DBC1 was localized between D9S1848 and AFMA239XA9. We have constructed an 840-kb sequence-ready contig composed of bacteriophage P1-derived artificial chromosomes (PACs), which encompasses DBC1. Clones were initially identified by screening a PAC library with markers localized to the region by physical mapping, and subsequently PAC end probes were used to complete the contig. This contig contains a minimum tiling path of six PAC clones between D9S1848 and AFMA239XA9. Three expressed sequence tags (ESTs) were mapped to the DBC1 region by screening 24 ESTs mapped to the surrounding area by radiation hybrids. One represented the gene for DBCCR1, a known candidate for DBC1, and the other two were novel. This contig and preliminary expression map form the basis for the identification of the bladder cancer tumor suppressor gene in this region. (C) 1999 Academic Press.