Genetically engineered biosynthetic pathways for nonnatural C60 carotenoids using C5-elongases and C50-cyclases in Escherichia coli
Genetically engineered biosynthetic pathways for nonnatural C60 carotenoids using C5-elongases and C50-cyclases in Escherichia coli
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DOI：
10.1038/s41598-019-39289-w
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发表时间：
2019-02-27
影响因子：
4.6
通讯作者：
Umeno, Daisuke
中科院分区：
文献类型：
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作者：
Li, Ling;Furubayashi, Maiko;Umeno, Daisuke
虽然大多数天然类胡萝卜素色素基于40-碳（C-40）骨架，但来自细菌的一些类胡萝卜素具有更大的C-50骨架，通过将两个异戊二烯单元（C-5）连接到C-40类胡萝卜素色素番茄红素的两侧来生物合成。随后的环化反应导致C-50类胡萝卜素的生产具有多样性和独特的骨架结构。为了生产更大的具有C-50 + C-5 + C-5 = C-60骨架的非天然新型类胡萝卜素，我们系统地共表达了来自各种细菌来源的天然C-50类胡萝卜素生物合成酶（番茄红素C-5-延伸酶和C-50-环化酶）以及大肠杆菌中实验室工程化的非天然C-50-番茄红素途径。在所测试的酶中，来自藤黄微球菌的延伸酶和环化酶对C-50-番茄红素表现出显著的活性，并产生新的类胡萝卜素C-60-黄黄素和C-60-肌黄质。此外，M.叶黄素延伸酶与棒状杆菌环化酶的反应导致C-60-肌黄质、C-60-肌原黄质和C-60-十肾上腺黄质的产生。
While the majority of the natural carotenoid pigments are based on 40-carbon (C-40) skeleton, some carotenoids from bacteria have larger C-50 skeleton, biosynthesized by attaching two isoprene units (C-5) to both sides of the C-40 carotenoid pigment lycopene. Subsequent cyclization reactions result in the production of C-50 carotenoids with diverse and unique skeletal structures. To produce even larger nonnatural novel carotenoids with C-50 + C-5 + C-5 = C-60 skeletons, we systematically coexpressed natural C-50 carotenoid biosynthetic enzymes (lycopene C-5-elongases and C-50-cyclases) from various bacterial sources together with the laboratory-engineered nonnatural C-50-lycopene pathway in Escherichia coli. Among the tested enzymes, the elongases and cyclases from Micrococcus luteus exhibited significant activity toward C-50-lycopene, and yielded the novel carotenoids C-60-flavuxanthin and C-60-sarcinaxanthin. Moreover, coexpression of M. luteus elongase with Corynebacterium cyclase resulted in the production of C-60-sarcinaxanthin, C-60-sarprenoxanthin, and C-60-decaprenoxanthin.