Not only the nature of peptide but also the characteristics of cell membrane determine the antimicrobial mechanism of a peptide
Not only the nature of peptide but also the characteristics of cell membrane determine the antimicrobial mechanism of a peptide
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DOI：
10.1034/j.1399-3011.1999.00051.x
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发表时间：
1999-05-01
期刊：
影响因子：
--
通讯作者：
Kim, SC
中科院分区：
文献类型：
--
作者：
Liang, JF;Kim, SC
本文研究了爪蟾抗菌素2、蟾蜍素II和多聚赖氨酸对大肠杆菌的抗菌作用机理。多聚赖氨酸抑制BL 21、AD 434和GroE（+）/DnaK（+）细胞生长，但不裂解细胞。Magainin 2对BL 21和ao 434细胞膜具有致孔活性，但在营养丰富的培养基中不能抑制GroE（+）/DnaK（+）的生长。Buforin II杀死BL 21和AD 434而不损伤细胞膜，裂解GroE+/DnaK+至死亡。一旦通过电穿孔将它们引入细胞中，所有三种肽都能够在低于其MICs. These结果的10倍的浓度下抑制细胞生长，表明肽的性质以及细胞膜的特性决定肽的抗微生物作用。
The mechanisms of antimicrobial actions of magainin 2, buforin II and poly L-lysine against various Escherichia coli strains were studied. Poly L-lysine inhibited BL21, AD 434 and GroE(+)/DnaK(+) growth without lysing the cell. Magainin 2 had a pore-forming activity on BL 21 and ao 434 membrane but could not inhibit the GroE(+)/DnaK(+) growth in a nutrient-rich medium. Buforin II, which killed BL21 and AD 434 without cell membrane damage, lysed GroE+/DnaK+ to death. Once they were introduced into the cell by electroporation, all three peptides were able to inhibit cell growth at concentrations of 10 times lower than their MICs. These results indicate that the nature of the peptide and also the characteristics of the cell membrane determine the antimicrobial actions of a peptide.