Anti-tumor and chemosensitization effects of Cryptotanshinone extracted from Salvia miltiorrhiza Bge. on ovarian cancer cells in vitro
Anti-tumor and chemosensitization effects of Cryptotanshinone extracted from Salvia miltiorrhiza Bge. on ovarian cancer cells in vitro
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DOI：
10.1016/j.jep.2017.04.026
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发表时间：
2017-06-09
影响因子：
5.4
通讯作者：
Li, Weiling
中科院分区：
文献类型：
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作者：
Jiang, Guoqiang;Liu, Jia;Li, Weiling
民族药理学相关性：隐丹参酮，从丹参根分离的天然化合物。（丹参），是一种常用的中药治疗高血压在一些国家。研究表明隐丹参酮可诱导癌细胞凋亡，并抑制癌细胞的迁移和侵袭。然而，隐丹参酮对卵巢癌的抗增殖和化疗增敏作用及其机制尚未完全阐明。研究目的：在这项研究中，我们评估了隐丹参酮对卵巢癌细胞的抑制作用，并探讨了潜在的分子机制。材料与方法：采用MTT法检测隐丹参酮和顺铂对卵巢癌A2780细胞的杀伤活性。流式细胞仪检测细胞凋亡。伤口愈合和transwell测定用于隐丹参酮处理的卵巢癌细胞的迁移和侵袭潜力评估。Western blot检测肿瘤转移和凋亡相关蛋白。结果：隐丹参酮可通过激活caspase级联反应诱导卵巢癌A2780细胞凋亡，并与顺铂联合作用后，细胞凋亡率明显降低。此外，伤口愈合和transwell实验显示隐丹参酮可以抑制卵巢癌细胞的迁移和侵袭，并显着抑制MMP-2和MMP-9的表达。结论：隐丹参酮对卵巢癌细胞具有明显的抗肿瘤作用，为隐丹参酮对卵巢癌细胞化疗增敏提供了新的研究结果。
Ethnopharmacological relevance: Cryptotanshinone, a natural compound isolated from the roots of Salvia miltiorrhiza Bge. (Danshen), is a commonly used traditional Chinese medicine to treat high blood pressure in some countries. It has been shown that Cryptotanshinone induces cancer cells apoptosis and impairs cell migration and invasion. However, the antiproliferation and chemosensitization effects of Cryptotanshinone on ovarian cancer and the underlying mechanism are not fully elucidated.Aim of study: In this study, we evaluated the inhibitory effect of Cryptotanshinone on ovarian cancer cells and explored the underlying molecular mechanism. Additionally, the chemosensitization potential of Cryptotanshinone was evaluated in combination with cisplatin.Materials and methods: MIT assay was used for cell viability assessment of ovarian cancer A2780 cells treated with Cryptotanshinone and/or cisplatin. Flow cytometry was used for apoptosis analysis. Wound healing and transwell assays were used for migratory and invasive potential assessment of Cryptotanshinone-treated ovarian cancer cells. Western blot was used to investigate proteins involved in the mechanisms for metastasis and apoptosis. gamma H2AX immunocytochemistry was used to detect DNA damage in A2780 cells exposed to Cryptotanshinone and/or cisplatin.Results: Cryptotanshinone significantly induced ovarian cancer A2780 cells apoptosis by activating caspase cascade. Additionally, wound healing and transwell assays revealed that Cryptotanshinone could suppress migration and invasion of ovarian cancer cells and dramatically inhibited MMP-2 and MMP-9 expression. Furthermore, Cryptotanshinone could sensitize A2780 cells to cisplatin treatment in a dose-dependent manner.Conclusion: Our data confirmed the anti-tumor effect of Cryptotanshinone on ovarian cancer cells and provided new findings that Cryptotanshinone could sensitize ovarian cancer cells to chemotherapy.