Combination use of anti-CD133 antibody and SSA lectin can effectively enrich cells with high tumorigenicity
Combination use of anti-CD133 antibody and SSA lectin can effectively enrich cells with high tumorigenicity
复制标题
DOI：
10.1111/j.1349-7006.2011.01923.x
复制
发表时间：
2011-06-01
期刊：
影响因子：
5.7
通讯作者：
Miyoshi, Eiji
中科院分区：
文献类型：
--
作者：
Moriwaki, Kenta;Okudo, Kumiko;Miyoshi, Eiji
聚糖在发育过程中表现出其结构的特征性变化，因此已被用作干细胞/祖细胞的标志物。然而，癌症干细胞（CSC）特有的聚糖结构仍然未知。在本研究中，我们研究了聚糖结构的CD133 + CD13 + CSC，这是最近发现有高CSC的能力，通过凝集素微阵列。MAL-1、SNA、SSA、TJA-1、ACG、ABA和MAH 7种唾液酸化聚糖识别凝集素对CD133 + CD13 + CSC的亲和力高于对CSC能力较低的CD133+细胞。此外，我们证明了从Huh7细胞中分离的CD133 + SSA+细胞在非肥胖糖尿病/严重联合免疫缺陷病（NOD/SCID）小鼠和无血清条件下的球体中形成肿瘤的能力显著高于CD133 + SSA-细胞。这些结果表明，肝CSC高度表达唾液酸化聚糖，SSA凝集素可用作分离CSC的工具。这项研究是第一份报告，以证明在CSC的特征聚糖结构，并指出一个新的方法，涉及凝集素分离CSC。（Cancer Sci 2011; 102：1164 - 1170）。
Glycans exhibit characteristic changes in their structures during development and thus have been used as markers for stem/progenitor cells. However, the glycan structures unique to cancer stem cells (CSC) remain unknown. In the present study, we examined glycan structures in CD133+CD13+ CSC, which were recently found to have a high CSC ability, by means of a lectin microarray. Seven sialylated glycan-recognizing lectins, MAL-I, SNA, SSA, TJA-I, ACG, ABA and MAH, showed higher affinity to CD133+CD13+ CSC than CD133+ cells with a lower CSC ability. In addition, we demonstrated that CD133+SSA+ cells isolated from Huh7 cells had a significantly higher ability to form tumors in non-obese diabetic/severe combined immunodeficiency disease (NOD/SCID) mice and spheres under serum-free conditions than CD133+SSA- cells. These results suggest that hepatic CSC highly express sialylated glycans and that SSA lectin can be used as a tool for isolating CSC. This study is the first report to demonstrate the characteristic glycan structures in CSC and to indicate a new methodology involving lectins for isolating CSC. (Cancer Sci 2011; 102: 1164-1170).