Engineering protein and cell adhesivity using PEO-terminated triblock polymers
Engineering protein and cell adhesivity using PEO-terminated triblock polymers
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DOI：
10.1002/jbm.10005
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发表时间：
2002-04-01
期刊：
影响因子：
--
通讯作者：
Bhatia, SN
中科院分区：
文献类型：
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作者：
Liu, VA;Jastromb, WE;Bhatia, SN
先前关于定制细胞培养环境的研究已经利用各种基于微制造的技术来控制粘附蛋白和随后的哺乳动物细胞的空间定位。其他人已经使用各种方法将非粘性的基于PEO的聚合物粘附在表面上以抑制蛋白质吸收和细胞粘附。在这项研究中，我们报告了一个良好的特点，商业上可获得的，PEO封端的三嵌段聚合物（普朗尼克（TM）F108）的应用程序，以创建各种生物材料，阻止细胞粘附长达4周的培养微图案的非粘附域。Pluronic可以使用微流体工具或微流控技术应用，并且可以吸附到各种常见表面，包括组织培养聚苯乙烯、甲基化玻璃、硅酮和聚乳酸-共-乙醇酸。还定量了Pluronic在胶原I存在下抑制细胞粘附的有效性。最后，这些图案化技术被推广到控制各种常见生物材料上的组织结构。这种用于微图案化PEO的简单方法，因此，蛋白质和细胞应该被证明是生物分子表面工程的有用工具。(C)2002年John Wiley Sons，Inc.
Previous studies on customizing cell culture environments have utilized a variety of microfabrication-based took to control the spatial localization of adhesive proteins and subsequently mammalian cells. Others have used various methods to immobilize nonadhesive PEO-based polymers on surfaces to inhibit protein absorption and cell adhesion. In this study, we report the application of a well-characterized, commercially available, PEO-terminated triblock polymer (Pluronic(TM) F108) to create micropatterned nonadhesive domains on a variety of biomaterials that deter cell adhesion for up to 4 weeks in culture. The Pluronic can be applied using microfluidic tools or photolithographic techniques, and can be adsorbed to a variety of common surfaces including tissue culture polystyrene, methylated glass, silicone, and polylactic-co-glycolic acid. The effectiveness of the Pluronic in inhibiting cell adhesion in the presence of collagen I is also quantified. Finally, these patterning techniques are generalized to control tissue organization on a variety of common biomaterials. This simple method for micropatterning PEO and, therefore, proteins and cells should prove useful as a tool for biomolecular surface engineering. (C) 2002 John Wiley Sons, Inc.