The forkhead-associated domain protein Cep170 interacts with polo-like kinase 1 and serves as a marker for mature centrioles
The forkhead-associated domain protein Cep170 interacts with polo-like kinase 1 and serves as a marker for mature centrioles
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DOI：
10.1091/mbc.e04-10-0939
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发表时间：
2005-03-01
影响因子：
3.3
通讯作者：
Nigg, EA
中科院分区：
文献类型：
--
作者：
Guarguaglini, G;Duncan, PI;Nigg, EA
我们报告的Cep170，叉头相关（FHA）结构域蛋白以前未知的功能的表征。Cep170在酵母双杂交筛选Polo样激酶1（Plk1）的相互作用物中被鉴定。在人类细胞中，Cep170在整个细胞周期中不断表达，但在有丝分裂期间磷酸化。它在体内与Plk 1相互作用，并可在体外被Plk 1磷酸化，表明它是该激酶的生理底物。过度表达和小干扰RNA（siRNA）介导的耗竭研究表明，Cep170在微管组织和细胞形态中的作用。Cep170在分裂间期与中心体结合，在有丝分裂期与纺锤体微管结合。如免疫电镜所示，Cep170与近远侧附属物，典型的成熟的母亲中心粒。因此，抗Cep170抗体在细胞周期的G1、S和早期G2期间仅染色一个中心粒，但在晚期G2期期间染色两个中心粒。我们发现，Cep170标记可以用来区分真正的中心粒过度复制从中心粒扩增，结果从中止细胞分裂。
We report the characterization of Cep170, a forkhead-associated (FHA) domain protein of previously unknown function. Cep170 was identified in a yeast two-hybrid screen for interactors of Polo-like kinase 1 (Plk1). In human cells, Cep170 is constantly expressed throughout the cell cycle but phosphorylated during mitosis. It interacts with Plk1 in vivo and can be phosphorylated by Plk1 in vitro, suggesting that it is a physiological substrate of this kinase. Both overexpression and small interfering RNA (siRNA)-mediated depletion studies suggest a role for Cep170 in microtuble organization and cell morphology. Cep170 associates with centrosomes during interphase and with spindle microtubules during mitosis. As shown by immunoelectron microscopy, Cep170 associates with subdistal appendages, typical of the mature mother centriole. Thus, anti-Cep170 antibodies stain only one centriole during G1, S, and early G2, but two centrioles during late G2 phase of the cell cycle. We show that Cep170 labeling can be used to discriminate bona fide centriole overduplication from centriole amplification that results from aborted cell division.