Visualization of polymercurimethane-labeled for bacteriophage in the scanning transmission electron microscope.
Visualization of polymercurimethane-labeled for bacteriophage in the scanning transmission electron microscope.
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在扫描透射电子显微镜中可视化聚合物库甲烷标记的噬菌体。
DOI：
10.1126/science.92053
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发表时间：
1979
期刊：
影响因子：
56.9
通讯作者：
J. Wall
中科院分区：
文献类型：
--
作者：
J. Lipka;S. Lippard;J. Wall
用四乙酰氧基汞甲烷（TAMM）或水甘氨酰甲硫氨酸铂（II）标记了fd噬菌体的2700个外壳蛋白。TAMM标记的样品在扫描透射电子显微镜下显示出由聚类产生的引人注目的亮点。质量的测量显示增加与分别向每个外壳蛋白添加四个汞原子或一个铂原子一致。
Each of the 2700 coat proteins of fd bacteriophage was labeled with tetrakis(acetoxymercuri)methane (TAMM) or aquoglycylmethionineplatinum(II). The TAMM-labeled specimens reveal striking bright spots in the scanning transmission electron microscope which arise from clustering. Measurements of mass show increases consistent with the addition of four mercury atoms or one platinum atom, respectively, to each coat protein.