Systematic Investigations of the ZF-HD Gene Family in Tobacco Reveal Their Multiple Roles in Abiotic Stresses
Systematic Investigations of the ZF-HD Gene Family in Tobacco Reveal Their Multiple Roles in Abiotic Stresses
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DOI：
10.3390/agronomy11030406
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发表时间：
2021-03-01
期刊：
影响因子：
3.7
通讯作者：
Sun, Yuhe
中科院分区：
文献类型：
--
作者：
Sun, Jinhao;Xie, Minmin;Sun, Yuhe
锌指同源结构域 (ZF-HD) 转录因子在植物生长和对环境胁迫的响应中发挥着重要作用。在这项研究中，根据拟南芥和番茄ZF-HD成员的系统发育分析，在烟草(Nicotiana tabacum L.)基因组中鉴定出的32个ZF-HD基因被分为六组。对基因结构和保守基序的检查揭示了每个亚组内相对保守的外显子/内含子结构和基序组织。此外，在这些基因的启动子区域还发现了各种与应激相关的元件。表达谱分析表明，NtZF-HD 基因在不同组织中表达，并且可能受到多种非生物胁迫的诱导。值得注意的是，NtZF-HD21 在响应干旱处理时高度表达。进行亚细胞定位分析和病毒诱导基因沉默（VIGS）实验来研究 NtZF-HD21 的潜在功能。亚细胞定位表明NtZF-HD21是核蛋白。此外，NtZF-HD21基因的基因沉默降低了烟草的抗旱性。这些发现为烟草中 NtZF-HD 基因的进一步生物学功能分析提供了见解。
Zinc finger homeodomain (ZF-HD) transcription factors play significant roles in plant growth and responses to environmental stresses. In this study, 32 ZF-HD genes identified in the tobacco (Nicotiana tabacum L.) genome were divided into six groups according to phylogenetic analysis with Arabidopsis and tomato ZF-HD members. An examination of gene structures and conserved motifs revealed the relatively conserved exon/intron structures and motif organization within each subgroup. In addition, various stress-related elements are found in the promoter region of these genes. The expression profiling analysis revealed that NtZF-HD genes expressed in different tissues and could be induced by several abiotic stresses. Notably, NtZF-HD21 was highly expressed in response to the drought treatments. Subcellular localization analysis and a virus-induced gene silencing (VIGS) experiment were performed to investigate the potential functions of NtZF-HD21. The subcellular localization indicated that NtZF-HD21 is a nuclear protein. Furthermore, gene silencing of the NtZF-HD21 gene reduced the drought resistance of tobacco. These findings provide insights for further biological functional analyses of the NtZF-HD genes in tobacco.