FERMENTATION OF XYLANS BY BUTYRIVIBRIO-FIBRISOLVENS AND OTHER RUMINAL BACTERIA
FERMENTATION OF XYLANS BY BUTYRIVIBRIO-FIBRISOLVENS AND OTHER RUMINAL BACTERIA
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DOI：
10.1128/aem.53.12.2849-2853.1987
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发表时间：
1987-12-01
影响因子：
4.4
通讯作者：
BOTHAST, RJ
中科院分区：
文献类型：
--
作者：
HESPELL, RB;WOLF, R;BOTHAST, RJ
研究了纤维丁状弧菌和其他瘤胃细菌(6种，18株)发酵麦草粗木聚糖和发酵落叶松木聚糖或燕麦汁木聚糖的能力。对液体培养的碳水化合物利用、细胞生长(蛋白质)和发酵产酸进行监测。B.纤维分离因子49、H17c、AcTF2和D1在一个或多个木聚糖上的生长情况几乎与它们在纤维二糖-麦芽糖上的生长情况一样好。B.纤维分离物12、R28、A38、X10C34、ARD22a和X6C61在木聚糖上表现出中等生长。以瘤胃拟杆菌B14、琥珀酸杆菌S85、白色瘤胃球菌7、黄色瘤胃球菌C94和FD1、糊精蔗糖弧菌22B为菌株，对木聚糖进行了部分发酵。所有测试的木聚糖似乎都含有一小部分碳水化合物，这些碳水化合物支持非木聚糖酶降解菌株的低水平生长，如瘤胃单胞菌HD4。与在己糖上生长相比，大多数菌株在木聚糖上生长时产生相同的发酵酸；然而，观察到纤维分离杆菌49和瘤胃单胞菌HD4的乳酸水平降低。纤维状芽孢杆菌AcTF2、49、H17c和D1的酶活性测定表明，木糖酶活性是细胞相关的，木聚糖酶活性主要与培养液有关。这些酶的表达模式在不同的菌株之间以及在菌株生长的碳源之间都不同。
The ability of Butyrivibrio fibrisolvens and other ruminal bacteria (6 species, 18 strains) to ferment a crude xylan from wheat straw or to ferment xylans from larchwood or oat spelts was studied. Liquid cultures were monitored for carbohydrate utilization, cell growth (protein), and fermentation acid production. B. fibrisolvens 49, H17c, AcTF2, and D1 grew almost as well on one or more of the xylans as they did on cellobiose-maltose. B. fibrisolvens 12, R28, A38, X10C34, ARD22a, and X6C61 exhibited moderate growth on xylans. Partial fermentation of xylans was observed with Bacteroides ruminicola B14, Bacteroides succinogenes S85, Ruminococcus albus 7, Ruminococcus flavefaciens C94 and FD1, and Succinivibrio dextrinosolvens 22B. All xylans tested appeared to have a small fraction of carbohydrate that supported low levels of growth of nonxylanolytic strains such as Selenomonas ruminantium HD4. Compared to growth on hexoses, the same array of fermentation acids was produced upon growth on xylans for most strains; however, reduced lactate levels were observed for B. fibrisolvens 49 and Selenomonas ruminantium HD4. Measurements of enzyme activities of B. fibrisolvens AcTF2, 49, H17c, and D1 indicated that the xylobiase activities were cell associated and that the xylanase activities were predominantly associated with the culture fluid. The pattern of expression of these enzymes varied both between strains and between the carbon sources on which the strains were grown.