Kinetic resolution of 2-hydroxybutanoate racemic mixtures by NAD-independent L-lactate dehydrogenase
Kinetic resolution of 2-hydroxybutanoate racemic mixtures by NAD-independent L-lactate dehydrogenase
复制标题
通过不依赖 NAD 的 L-乳酸脱氢酶动力学拆分 2-羟基丁酸酯外消旋混合物
DOI：
10.1016/j.biortech.2011.01.003
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发表时间：
2011-04-01
影响因子：
11.4
通讯作者：
Xu, Ping
中科院分区：
文献类型：
--
作者：
Gao, Chao;Zhang, Wen;Xu, Ping
光学活性D-2-羟基丁酸酯是药物和可生物降解聚2-羟基丁酸酯的重要构筑中间体。外消旋2-羟基丁酸酯的动力学拆分可能是生产D-2-羟基丁酸酯的一种绿色和理想的替代方法。在这项工作中，D-2-羟基丁酸在高浓度(0.197 M)和高对映体过量(99.1%)生产L-乳酸脱氢酶(L-iLDH)的生物催化剂。另一种重要的中间体2-氧代丁酸酯在高浓度(0.193 M)下联产。用D301大孔阴离子交换树脂进行简单交换，将D-2-羟基丁酸酯从生物转化体系中分离出来，回收率高达84.7%。(C)2011爱思唯尔有限公司。保留所有权利。
Optically active D-2-hydroxybutanoate is an important building block intermediate for medicines and biodegradable poly(2-hydroxybutanoate). Kinetic resolution of racemic 2-hydroxybutanoate may be a green and desirable alternative for D-2-hydroxybutanoate production. In this work, D-2-hydroxybutanoate at a high concentration (0.197 M) and a high enantiomeric excess (99.1%) was produced by an NAD-independent L-lactate dehydrogenase (L-iLDH) containing biocatalyst. 2-Oxobutanoate, another important intermediate, was co-produced at a high concentration (0.193 M). Using a simpleton exchange process with the macroporous anion exchange resin D301, D-2-hydroxybutanoate was separated from the biotransformation system with a high recovery of 84.7%. (C) 2011 Elsevier Ltd. All rights reserved.