There exist at least 30 human G-protein-coupled receptors with long Ser/Thr-rich N-termini
There exist at least 30 human G-protein-coupled receptors with long Ser/Thr-rich N-termini
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DOI：
10.1016/s0006-291x(03)00026-3
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发表时间：
2003-02-14
影响因子：
3.1
通讯作者：
Schiöth, HB
中科院分区：
文献类型：
--
作者：
Fredriksson, R;Gloriam, DEI;Schiöth, HB
我们报告了通过在人类基因组数据库中搜索发现的人类 G 蛋白偶联受体 (GPCR) 超家族的六个新成员，称为 GPR123、GPR124、GPR125、GPR126、GPR127 和 GPR128。系统发育分析表明，这些是具有长 N 末端的 GPCR 家族的其他成员，以前称为 EGF-7TM、LNB-7TM、B2 或 LN-7TM，表明人类基因组中至少存在 30 个此类 GPCR。其中三个受体形成了自己的系统发育簇，而另外两个位置与先前报道的 HE6 和 GPR56 (TM7XN1) 以及一个与 EMR1-3 位于一个簇中。除 GPR123 外，所有新型受体的 N 末端均具有 GPS 结构域，以及形成粘蛋白样茎的长 Ser/Thr 丰富区域。 GPR124 和 GPR125 具有富含亮氨酸重复序列 (LRR)、免疫球蛋白 (Ig) 结构域和激素结合结构域 (HBD)。 Ig 结构域与胃动素和肌联蛋白相似，而 LRR 结构域与 LRIG1 和 SLIT1-2 相似。 GPR127 具有一个 EGF 结构域，而 GPR126 和 GPR128 不包含在 GPS 结构域之外的其他蛋白质中容易识别的结构域。我们发现大多数受体的几个人类 EST 序列显示出差异表达模式，这可能表明其中一些受体参与中枢功能，而其他受体更可能在免疫或生殖系统中发挥作用。 (C) 2003 年爱思唯尔科学（美国）。版权所有。
We report six novel members of the superfamily of human G-protein coupled receptors (GPCRs) found by searches in the human genome databases, termed GPR123, GPR124, GPR125, GPR126, GPR127, and GPR128. Phylogenetic analysis demonstrates that these are additional members of the family of GPCRs with long N-termini, previously termed EGF-7TM, LNB-7TM, B2 or LN-7TM, showing that there exist at least 30 such GPCRs in the human genome. Three of these receptors form their own phylogenetic cluster, while two other places in a cluster with the previously reported HE6 and GPR56 (TM7XN1) and one with EMR1-3. All the novel receptors have a GPS domain in their N-terminus, except GPR123, as well as long Ser/Thr rich regions forming mucin-like stalks. GPR124 and GPR125 have a leucine rich repeat (LRR), an immunoglobulin (Ig) domain, and a hormone-binding domain (HBD). The Ig domain shows similarities to motilin and titin, while the LRR domain shows similarities to LRIG1 and SLIT1-2. GPR127 has one EGF domain while GPR126 and GPR128 do not contain domains that are readily recognized in other proteins beyond the GPS domain. We found several human EST sequences for most of the receptors showing differential expression patterns, which may indicate that some of these receptors participate in central functions while others are more likely to have a role in the immune or reproductive systems. (C) 2003 Elsevier Science (USA). All rights reserved.