Simple microfluidic device for detecting the negative dielectrophoresis of DNA labeled microbeads
Simple microfluidic device for detecting the negative dielectrophoresis of DNA labeled microbeads
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DOI：
10.1063/1.5124419
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发表时间：
2019-11-01
期刊：
影响因子：
3.2
通讯作者：
Suehiro, Junya
中科院分区：
文献类型：
--
作者：
Nakano, Michihiko;Ding, Zhenhao;Suehiro, Junya
我们提出了一种新的微流控装置，可用于确定的变化，在负介电电泳（n-DEP）的电介质微珠时，少量的DNA附着到他们。我们以前提出了一种DNA检测方法的基础上的DEP的变化引起的DNA的附着的微珠。当靶DNA附着于具有n-DEP性质的微珠时，DEP从负变为正。这是因为DNA的电荷增加了微珠的表面电导。因此，只有DNA标记的微珠被正DEP吸引到微电极。捕获的DNA标记的微珠可以通过介电泳阻抗测量来计数。需要大量的DNA（约105个DNA分子）将DEP从阴性变为阳性。尽管这种方法可以与DNA扩增相结合，但减少所需的DNA量可以帮助我们缩短反应时间。在本研究中，我们的目标是通过确定n-DEP变化的变化来检测少于105个DNA分子的DNA。为了实现这一点，我们提出了一个简单的微流控装置，包括一个单一的微通道和一对微电极。数值模拟表明，该装置可以识别与少量DNA附着相对应的微珠的n-DEP的微小变化。在实际实验中，所制造的装置区分每个微珠10-1000个DNA分子。该方法与DNA扩增技术相结合，是一种快速简便的DNA检测方法。由AIP Publishing授权出版。
We propose a new microfluidic device that can be used to determine the change in the negative dielectrophoresis (n-DEP) of dielectric microbeads when a small amount of DNA is attached to them. We previously proposed a DNA detection method based on changes in the DEP of microbeads induced by the attachment of DNA. When target DNA is attached to the microbeads having n-DEP property, the DEP changes from negative to positive. This occurs because electric charges of the DNA increase the surface conductance of the microbeads. Thus, only the DNA-labeled microbeads are attracted to a microelectrode by positive DEP. The trapped DNA-labeled microbeads can be counted by dielectrophoretic impedance measurements. A large amount of DNA (approximately 105 DNA molecules) is required to change the DEP from negative to positive. Even though this method can be combined with DNA amplification, reducing the amount of DNA required can help us to shorten the reaction time. In this study, we aimed to detect DNA less than 105 DNA molecules by determining the change in the n-DEP change. To achieve this, we proposed a simple microfluidic device consisting of a single microchannel and a single pair of microelectrodes. Numerical simulations revealed that the device can identify the slight change in the n-DEP of the microbeads corresponding to the attachment of a small amount of DNA. In practical experiments, the fabricated device distinguished 10-1000 DNA molecules per microbead. This method represents a fast and easy method of DNA detection when combined with DNA amplification techniques. Published under license by AIP Publishing.