Remarkable inter- and intra-species complexity of conotoxins revealed by LC/MS
Remarkable inter- and intra-species complexity of conotoxins revealed by LC/MS
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DOI：
10.1016/j.peptides.2009.03.019
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发表时间：
2009-07-01
期刊：
影响因子：
3
通讯作者：
Lewis, Richard J.
中科院分区：
文献类型：
--
作者：
Davis, Jasmine;Jones, Alun;Lewis, Richard J.
锥形蜗牛进化出了各种各样的毒液肽，作为快速固定猎物和防御的进化策略。早期研究估计每个物种有大约 100 种锥肽。在本研究中，我们优化了液相色谱和电喷雾电离质谱法，用于检测粗毒液中的锥肽，以表征纺织锥虫、帝国锥虫和马尔莫雷伞锥虫个体样本的毒液中存在的锥肽。使用这种方法，我们将毒液肽的预测数量扩大了 10 倍，估计每个物种有 1000-1900 个锥肽。我们的调查还揭示了令人惊讶的高水平物种内变异，这将锥螺与包括蜘蛛和蝎子在内的其他有毒物种区分开来。鉴于这种固有的多样性和变异性，需要更灵敏的生物测定和测序技术来充分探索芋螺毒素的生物活性。 Crown 版权所有 (C) 2009 由 Elsevier Inc. 出版。保留所有权利。
Cone snails have evolved an assortment of venom peptides as an evolutionary strategy for rapid prey immobilization and defence. Earlier studies estimated similar to 100 conopeptides per species. In this study we optimized liquid chromatography and electrospray ionization mass spectrometry for the detection of conopeptides in crude venom to characterize conopeptides present in the venom of individual specimens of Conus textile, C. imperialis and C. marmoreus. Using this approach, we have expanded the predicted number of venom peptides 10-fold to an estimate of 1000-1900 conopeptides per species. Our investigation has also revealed a surprisingly high level of intra-species variation that distinguishes cone snails from other venomous species including spiders and scorpions. Given this inherent diversity and variability, more sensitive bioassays and sequencing techniques will be required to fully explore conotoxin bioactivity. Crown Copyright (C) 2009 Published by Elsevier Inc. All rights reserved.