Integrated analysis of miRNA and mRNA expression profiles in tilapia gonads at an early stage of sex differentiation.
Integrated analysis of miRNA and mRNA expression profiles in tilapia gonads at an early stage of sex differentiation.
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罗非鱼性腺性别分化早期 miRNA 和 mRNA 表达谱的综合分析
DOI：
10.1186/s12864-016-2636-z
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发表时间：
2016-05-04
期刊：
影响因子：
4.4
通讯作者：
Wang D
中科院分区：
文献类型：
--
作者：
Tao W;Sun L;Shi H;Cheng Y;Jiang D;Fu B;Conte MA;Gammerdinger WJ;Kocher TD;Wang D
研究背景MicroRNAs（miRNAs）是生物过程中对基因表达和蛋白质翻译起重要作用的第二个调控网络。然而，miRNAs在鱼类性别分化早期阶段的可能作用还不清楚。在这项研究中，我们进行了综合分析的miRNA和mRNA的表达谱，以探讨其可能的调控模式在性别分化的关键阶段在罗非鱼results我们确定了279前miRNA基因，这是高度保守的其他鱼类基因组。通过小RNA文库测序，我们在罗非鱼性腺中鉴定出635个成熟miRNAs，其中62个miRNAs在XX性腺中高表达，49个miRNAs在XY性腺中高表达。这些性别偏向的miRNA的预测靶点（例如，miR-9、miR-21、miR-30 a、miR-96、miR-200 b、miR-212和miR-7977）包括编码类固醇生成途径中的关键酶（Cyp 11 a1、Hsd 3b、Cyp 19 a1 a、Hsd 11b）和参与脊椎动物性别分化的关键分子（Foxl 2、Amh、Star 1、Sf 1、Dmrt 1和Gsdf）的基因。这些基因在5日龄时在罗非鱼性腺中的表达也表现出性别偏向性。一些miRNA（例如，miR-96和miR-737）靶向参与类固醇合成的多个基因，提示罗非鱼早期性别分化过程中存在复杂的miRNA调控网络.ConclusionsThe most miRNAs的序列和表达模式在鱼类中是保守的，表明脊椎动物miRNAs的基本功能可能有着共同的进化起源.通过对罗非鱼XX和XY性腺分子性别分化早期阶段的miRNA和mRNA的综合分析，发现了差异表达的miRNA及其可能的靶基因，为深入研究鱼类性别决定和分化的分子调控网络奠定了基础。
BackgroundMicroRNAs (miRNAs) represent a second regulatory network that has important effects on gene expression and protein translation during biological process. However, the possible role of miRNAs in the early stages of fish sex differentiation is not well understood. In this study, we carried an integrated analysis of miRNA and mRNA expression profiles to explore their possibly regulatory patterns at the critical stage of sex differentiation in tilapia.ResultsWe identified 279 pre-miRNA genes in tilapia genome, which were highly conserved in other fish species. Based on small RNA library sequencing, we identified 635 mature miRNAs in tilapia gonads, in which 62 and 49 miRNAs showed higher expression in XX and XY gonads, respectively. The predicted targets of these sex-biased miRNAs (e.g., miR-9, miR-21, miR-30a, miR-96, miR-200b, miR-212 and miR-7977) included genes encoding key enzymes in steroidogenic pathways (Cyp11a1,Hsd3b,Cyp19a1a,Hsd11b) and key molecules involved in vertebrate sex differentiation (Foxl2,Amh,Star1,Sf1,Dmrt1, andGsdf). These genes also showed sex-biased expression in tilapia gonads at 5 dah. Some miRNAs (e.g., miR-96 and miR-737) targeted multiple genes involved in steroid synthesis, suggesting a complex miRNA regulatory network during early sex differentiation in this fish.ConclusionsThe sequence and expression patterns of most miRNAs in tilapia are conserved in fishes, indicating the basic functions of vertebrate miRNAs might share a common evolutionary origin. This comprehensive analysis of miRNA and mRNA at the early stage of molecular sex differentiation in tilapia XX and XY gonads lead to the discovery of differentially expressed miRNAs and their putative targets, which will facilitate studies of the regulatory network of molecular sex determination and differentiation in fishes.