NITROUS-OXIDE PRODUCTION BY ORGANISMS OTHER THAN NITRIFIERS OR DENITRIFIERS
NITROUS-OXIDE PRODUCTION BY ORGANISMS OTHER THAN NITRIFIERS OR DENITRIFIERS
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DOI：
10.1128/aem.44.6.1342-1348.1982
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发表时间：
1982-01-01
影响因子：
4.4
通讯作者：
TIEDJE, JM
中科院分区：
文献类型：
--
作者：
BLEAKLEY, BH;TIEDJE, JM
异养细菌、酵母菌、真菌、植物和动物的呼气可能是N2O的来源。从NO3-产生N2O但不消耗N2O的微生物包括：所有被检查的N3-呼吸细菌(大肠杆菌、沙雷氏菌、克雷伯氏菌、肠杆菌、欧文氏杆菌和芽孢杆菌)；1种被检查的同化N3-还原细菌(棕色固氮杆菌)，但不包括固氮菌大细胞基因或不动杆菌；以及一些但不是全部的同化N3-还原酵母菌和真菌(Hansenula、Rhodotorula、曲霉、交链孢霉和镰刀菌的菌株)。硝酸根还原专性厌氧菌KDHS2不产生N2O。N2O的产生只发生在固定相。呼吸N3的细菌产生的N2O比其他微生物多得多，N2O的产量在3.5mMN3-的3-36%之间。N2O的产生显然不受NH4+的调节，仅限于好氧或厌氧条件下。植物似乎没有产生N2O，尽管N2O来自一些受损的植物顶部，可能是由于微生物的生长。人们在呼吸中发现，空气中N2O的浓度高于周围水平，而且在进食高硝酸盐食物后，N2O的浓度似乎会增加。
Heterotrophic bacteria, yeasts, fungi, plants and animal breath were investigated as possible sources of N2O. Microbes producing N2O from NO3- but not consuming it included: all of the NO3--respiring bacteria examined (strains of Escherichia, Serratia, Klebsiella, Enterobacter, Erwinia and Bacillus); 1 of the assimilatory NO3--reducing bacteria examined (Azotobacter vinelandii), but not Azotobacter macrocytogenes or Acinetobacter sp.; and some but not all of the assimilatory NO3--reducing yeasts and fungi (strains of Hansenula, Rhodotorula, Aspergillus, Alternaria and Fusarium). The NO3--reducing obligate anaerobe Clostridium KDHS2 did not produce N2O. Production of N2O occurred only in stationary phase. The NO3- respiring bacteria produced much more N2O than the other organisms; with yields of N2O ranging from 3-36% of 3.5 mM NO3-. Production of N2O was apparently not regulated by NH4+ and was restricted to aerobic or anaerobic conditions. Plants did not appear to produce N2O, although N2O arose from some damaged plant tops, probably due to microbial growth. Concentrations of N2O above the ambient level in the atmosphere were found in human breath and appeared to increase after a meal of high-nitrate food.