On-chip incubation system for long-term microfluidic cell culture
On-chip incubation system for long-term microfluidic cell culture
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DOI：
10.1109/iembs.2011.6092073
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发表时间：
2011-12
期刊：
影响因子：
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通讯作者：
Atsushi Takano;Tomohisa Ogawa;Masato Tanaka;N. Futai
中科院分区：
文献类型：
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作者：
Atsushi Takano;Tomohisa Ogawa;Masato Tanaka;N. Futai
我们演示了微流体细胞培养芯片与盲文针驱动泵的使用，能够进行片上 CO2 孵化，不需要外部室或气体供应。所提出的芯片由聚（二甲基硅氧烷）（PDMS）制成的微流控芯片组成，倒装在载玻片上，其中包含一对嵌套的细胞培养基储库和水套，由可渗透的 PDMS 壁绝缘。通过使用 0.8 M 碳酸氢钠和 65 mM 碳酸钠作为水套并放置在 37 °C 表面上，芯片在至少 24 小时内保持重量克分子渗透压浓度变化，并且介质储存器中的 pCO2 稳定在 < 3 mmol/kg 和 5.0% ± 0.2% 范围内。通过在倒置显微镜下对 CV-1 上皮细胞进行微流体培养至少 12 天，证明了孵化能力。
We demonstrate the use of a microfluidic cell culture chip with Braille pin-driven pumping, capable of on-chip CO2 incubation that does not require an external chamber or gas supply. The proposed chip consists of a poly(dimethylsiloxane)(PDMS)-made microfluidic chip, flip-mounted on a glass slide, that contains a nested pair of cell culture media reservoirs and water-jacket, insulated by a permeable PDMS wall. By using 0.8 M sodium bicarbonate with 65 mM sodium carbonate as the water-jacket and placing on a 37 °C surface, the chip maintained osmolality shift and the pCO2 in the media reservoir stabilized within < 3 mmol/kg and 5.0% ± 0.2% over at least 24 hours. The incubation capabilities were demonstrated through microfluidic culture of CV-1 epithelial cells under an inverted microscope for at least 12 days.