LIPID-ACCUMULATION IN THE MAJOR SALIVARY-GLANDS OF STREPTOZOTOCIN-DIABETIC RATS
LIPID-ACCUMULATION IN THE MAJOR SALIVARY-GLANDS OF STREPTOZOTOCIN-DIABETIC RATS
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DOI：
10.1016/0003-9969(86)90021-x
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发表时间：
1986-01-01
影响因子：
3
通讯作者：
GARRETT, JR
中科院分区：
文献类型：
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作者：
ANDERSON, LC;GARRETT, JR
诱导糖尿病四五个月后，通过戊二醛固定或在干冰冷却的己烷中快速冷冻来保存雄性Wistar大鼠的唾液组织。固定的组织要么被处理并包埋用于光学和电子显微镜检查，要么被冷冻并与未固定的组织一起切片并用油红0或通过钙脂肪酶方法染色。所有糖尿病腺体的细胞内脂质都比对照腺体多得多。实质细胞内的脂质积累随腺体类型的不同而变化，并且在血清葡萄糖水平最高的动物中更为明显。腮腺和舌下腺的浆液细胞积聚的脂质最多；下颌下腺的浆液粘液腺泡细胞中存在较少量；舌下腺的粘液腺泡细胞中很少或没有。横纹管、颗粒管或排泄管中没有脂质。组织化学染色表明，细胞内脂质主要是甘油三酯，其可能通过增加摄取以用作能量来源或通过减少分泌颗粒和质膜材料的合成而积累。
Four-five months after the induction of diabetes, salivary tissues of male Wistar rats were preserved by glutaraldehyde fixation or rapid freezing in dry-ice cooled hexane. Fixed tissues were either processed and embedded for light and electron microscopy, or frozen and, together with unfixed tissues, sectioned and stained with Oil Red 0 or by the calcium-lipase method. All diabetic glands had considerably more intracellular lipid than control ones. Lipid accumulation within parenchymal cells varied with the type of gland, and was more pronounced in animals with the highest serum-glucose levels. Serous cells of parotid and sublingual glands accumulated the greatest amount of lipid; lesser amounts were present in seromucous acinar cells of submandibular glands; little or none in mucous acinar cells of sublingual glands. There was no lipid in striated, granular or excretory ducts. Histochemical staining suggested that the intracellular lipid was mainly triglyceride which may accumulate by increased uptake for use as an energy source, or by decreased use in the synthesis of secretory granule and plasma-membrane material.