HIGHLY SENSITIVE SILVER STAIN FOR DETECTING PROTEINS AND PEPTIDES IN POLYACRYLAMIDE GELS
HIGHLY SENSITIVE SILVER STAIN FOR DETECTING PROTEINS AND PEPTIDES IN POLYACRYLAMIDE GELS
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DOI：
10.1016/0003-2697(79)90732-2
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发表时间：
1979-01-01
影响因子：
2.9
通讯作者：
SHIFRIN, S
中科院分区：
文献类型：
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作者：
SWITZER, RC;MERRIL, CR;SHIFRIN, S
我们开发了一种高灵敏度的染色剂，用于在聚丙烯酰胺凝胶中显示蛋白质。我们对de Olmos神经铜银染色程序的修改比传统的考马斯蓝染色灵敏100倍（例如，血清白蛋白的检测值分别为0.38 ng/mm 2和38 ng/mm 2），与暴露5天后14 C-甲基化蛋白放射自显影获得的灵敏度相当。这种银染色将特别适用于分析来自组织的蛋白质的模式，其中获得检测次要蛋白质组分所必需的同位素标记的高比活性是昂贵的，技术上困难的，或者如在人类中是被禁止的。在未浓缩脑脊液等材料的初步结果中，银染色显示了考马斯亮蓝不可见的复杂蛋白质模式。
We have developed a highly sensitive stain for visualizing proteins in polyacrylamide gels. Our modification of the procedure for de Olmos' neural, cupric-silver stain is 100 times more sensitive than the conventional Coomassie blue stain (e.g., detection of 0.38 vs 38 ng/mm2of serum albumin), and is comparable to the sensitivity attained with an autoradiogram of14C-methylated proteins following a 5-day exposure. This silver stain will be especially useful for analysis of patterns of proteins from tissue where attainment of the high specific activity of isotope labeling which is necessary to detect minor protein components is expensive, technically difficult or, as in humans, prohibited. In preliminary results with material such as unconcentrated cerebrospinal fluid, the silver stain revealed a complex pattern of proteins not visible with Coomassie blue.