Effect of alpha-lipoic acid on radiation-induced small intestine injury in mice.
Effect of alpha-lipoic acid on radiation-induced small intestine injury in mice.
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DOI：
10.18632/oncotarget.7874
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发表时间：
2016-03-22
期刊：
影响因子：
--
通讯作者：
Kang KM
中科院分区：
文献类型：
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作者：
Jeong BK;Song JH;Jeong H;Choi HS;Jung JH;Hahm JR;Woo SH;Jung MH;Choi BH;Kim JH;Kang KM
放射治疗对于实体瘤患者来说是一种非常有效的治疗方法。然而，它会导致正常组织损伤和炎症。在这里，我们研究了α-硫辛酸（ALA）作为辐射防护剂对小鼠模型小肠的影响。整个腹部均匀照射，总剂量为15Gy。在辐射前，小鼠接受 ALA（100 mg/kg，腹腔注射 [i.p.]）或生理盐水（等体积，i.p.）治疗，剂量为 100 mg/kg/天，持续 3 天。评估体重、食物摄入量、组织病理学和生化参数。在放疗 (RT) 组和 ALA + RT 组之间观察到体重和食物摄入量存在显着差异。此外，ALA + RT 组的隐窝细胞数量更高。与 RT 组相比，ALA + RT 组炎症减轻，恢复时间缩短。与 RT 组相比，ALA + RT 组炎症相关因子（即磷酸化核因子 kappa B 和基质金属蛋白酶-9）和丝裂原激活蛋白激酶的水平显着降低。放疗前进行 ALA 治疗可通过减少炎症、氧化应激和细胞死亡来降低放射性肠炎的严重程度和持续时间。
Radiation therapy is a highly effective treatment for patients with solid tumors. However, it can cause damage and inflammation in normal tissues. Here, we investigated the effects of alpha-lipoic acid (ALA) as radioprotection agent for the small intestine in a mouse model. Whole abdomen was evenly irradiated with total a dose of 15 Gy. Mice were treated with either ALA (100 mg/kg, intraperitoneal injection [i.p.]) or saline (equal volume, i.p.) the prior to radiation as 100 mg/kg/day for 3 days. Body weight, food intake, histopathology, and biochemical parameters were evaluated. Significant differences in body weight and food intake were observed between the radiation (RT) and ALA + RT groups. Moreover, the number of crypt cells was higher in the ALA + RT group. Inflammation was decreased and recovery time was shortened in the ALA + RT group compared with the RT group. The levels of inflammation-related factors (i.e., phosphorylated nuclear factor kappa B and matrix metalloproteinase-9) and mitogen-activated protein kinases were significantly decreased in the ALA + RT group compared with those in the RT group. ALA treatment prior to radiation decreases the severity and duration of radiation-induced enteritis by reducing inflammation, oxidative stress, and cell death.