Carbonic anhydrases in meningiomas: association of endothelial carbonic anhydrase II with aggressive tumor features Laboratory investigation
Carbonic anhydrases in meningiomas: association of endothelial carbonic anhydrase II with aggressive tumor features Laboratory investigation
复制标题
DOI：
10.3171/2008.10.17672
复制
发表时间：
2009-09-01
影响因子：
4.1
通讯作者：
Haapasalo, Hannu
中科院分区：
文献类型：
--
作者：
Korhonen, Katariina;Parkkila, Anna-Kaisa;Haapasalo, Hannu
目的。碳酸酐酶 (CA) II 和 IX 是参与 pH 稳态的酶，并且已被证明在多种类型的癌症中表达上调。在这项研究中，作者评估了脑膜瘤中 CA II 和 IX 的表达，并评估了它们与患者年龄、肿瘤类型和级别、肿瘤性激素受体状态的关系。肿瘤细胞增殖和肿瘤复发。方法。这项研究是在 1989 年至 1999 年间在坦佩雷大学医院接受脑膜瘤手术的连续患者中进行的。使用组织微阵列技术和特异性抗体对 CA II 和 IX 的表达进行免疫组织化学研究。结果。在 443 个原发性肿瘤样本和 67 个复发性肿瘤样本中评估了 CA II 和 IX 的免疫组织学染色。在这些样本中，455 个是良性的（WHO I 级），49 个非典型样本（II 级）和 6 个恶性样本（III 级）。 14.8% 肿瘤中的内皮细胞 CA II 染色呈阳性。 11.6% 的病例肿瘤细胞 CA IX 呈阳性。内皮 CA H 表达与组织学分级的增加相关 (p = 0.002)，CA II+ 病例的肿瘤增殖率高于 CA II- 病例 (p = 0.002)。雄激素受体阴性肿瘤 CA II+ 的发生率显着高于雄激素受体阳性肿瘤 (p = 0.001)。未发现与 CA IX 酶的关联。结论。内皮细胞碳酸酐酶 II 阳性与细胞增殖和恶性程度相关。这些结果表明CA II表达与脑膜瘤的恶性进展相关，因此可能成为抗癌治疗的靶分子。 （DOI：10.3171/2008.10.17672）
Object. Carbonic anhydrase (CA) II and IX are enzymes involved in pH homeostasis and have been shown to be upregulated in several types of cancer. In this Study, the authors evaluate the expression of CA II and IX in meningiomas and assess their relationship to patient age, tumor type and grade, tumor sex hormone receptor status. tumor cell proliferation, and tumor recurrence.Methods. This study was conducted in consecutive patients who underwent meningioma surgeries at Tampere University Hospital between 1989 and 1999. The expression of CA II and IX was studied immunohistochemically using a tissue microarray technique and specific antibodies.Results. Immunohistological staining with CA II and IX was assessed in 443 primary and 67 recurrent tumor specimens. Of these samples, 455 were benign (WHO Grade I), 49 atypical (Grade II), and 6 malignant (Grade III). Endothelial cells in 14.8% of the tumors stained positively for CA II. Tumor cells were positive for CA IX in 11.6% of the cases. Endothelial CA H expression correlated with increasing histological grade (p = 0.002), and tumor proliferation rates were higher in CA II+ versus CA II- cases (p = 0.002). Androgen receptor-negative tumors were found to be CA II+ significantly more often than androgen receptor-positive tumors (p = 0.001). No associations were found with the CA IX enzyme.Conclusions. Carbonic anhydrase II positivity in the endothelium was associated with cell proliferation and malignancy grade. These results Suggest that CA II expression is associated with malignant progression of meningiomas and could thus be a target molecule for anticancer therapy. (DOI: 10.3171/2008.10.17672)