Complete nucleotide sequence of pKP96, a 67 850 bp multiresistance plasmid encoding qnrA1, aac(6')-Ib-cr and blaCTX-M-24 from Klebsiella pneumoniae
Complete nucleotide sequence of pKP96, a 67 850 bp multiresistance plasmid encoding qnrA1, aac(6')-Ib-cr and blaCTX-M-24 from Klebsiella pneumoniae
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DOI：
10.1093/jac/dkn397
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发表时间：
2008-12-01
影响因子：
5.2
通讯作者：
Li, Lanjuan
中科院分区：
文献类型：
--
作者：
Shen, Ping;Jiang, Yan;Li, Lanjuan
对肺炎克雷伯菌多重耐药质粒pKP 96进行全基因组测序，分析其遗传环境和耐药基因的分布，并采用全基因组鸟枪法测定其全基因组序列。使用Etests测定13种抗菌药物的MIC。在液体培养基中进行接合实验。pKP 96是一个环状封闭的67 850 bp的多抗性质粒与IncN不相容组。根据已完成序列的注释鉴定了70个推定基因。质粒的骨架区域，包括接合转移和质粒复制区域，显示与IncN质粒R46的91%同一性。质粒pKP 96是一个从不同来源水平获得多个耐药决定簇的嵌合体，其中qnrA 1、aac（6 '）-Ib-cr和bla（CTX-M-24）是几个移动的元件。它可能是通过整合或重组的逐步事件从类似于R46的祖先质粒进化而来的。
The multiresistance plasmid pKP96 from Klebsiella pneumoniae was sequenced completely and analysed concerning its genetic environment and distributing of antimicrobial resistance genes.The complete sequence of the plasmid was determined using a whole-genome shotgun approach. MICs of 13 antimicrobial agents were determined using Etests. A conjugation experiment was performed in liquid medium.pKP96 is a circularly closed 67 850 bp multiresistance plasmid with an IncN incompatibility group. Seventy putative genes were identified according to the annotation of the finished sequence. The backbone region of the plasmid, comprising the conjugal transfer and plasmid replication regions, showed 91% identity to the IncN plasmid R46. Several mobile elements were found to be inserted into pKP96 together with antimicrobial resistance genes, including qnrA1, aac(6')-Ib-cr and bla(CTX-M-24).Plasmid pKP96 is a chimera that has acquired its multiple antimicrobial resistance determinants horizontally from different sources. It may have evolved from an ancestor plasmid similar to R46 through the stepwise events of integration or recombination.