Antibody response in dogs experimentally infected with Leishmania infantum: Infection course antigen markers
Antibody response in dogs experimentally infected with Leishmania infantum: Infection course antigen markers
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DOI：
10.1006/expr.1996.0018
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发表时间：
1996-03-01
影响因子：
2.1
通讯作者：
Alunda, JM
中科院分区：
文献类型：
--
作者：
Carrera, L;Fermin, ML;Alunda, JM
用婴儿利什曼原虫无鞭毛体感染5月龄比格犬，饲养14个月。感染过程通过临床检查、血清蛋白变化以及通过间接免疫荧光试验和蛋白质印迹（WE）估计的抗利什曼原虫的特异性抗体水平来监测。感染的动物在感染后2-3个月（p.i.）与γ-球蛋白组分有关。特异性抗体滴度与血清蛋白升高一致，免疫荧光值在感染后3个月达到1：800以上。用L.在研究的大部分时间内，婴儿前鞭毛体蛋白显示出对50-57、42和29 kDa的免疫显性抗原的强烈应答，免疫荧光滴度超过1：100，此外，该应答在感染的狗中是显著均匀的。免疫反应性模式显示与时间相关的变化;对29和50-57 kDa的反应是非常早的，其次是42，76和86 kDa左右的反应。此外，34-35.4和26 kDa左右肽的识别仅限于实验感染的急性期。在自然感染犬中获得的初步结果似乎支持WB的预测值。(C)出版社：Academic Press，Inc.
Five-month-old beagle dogs were experimentally infected with amastigotes of Leishmania infantum and kept for 14 months. infection course was monitored by clinical examination, serum protein variations, and levels of specific antibodies against Leishmania estimated by indirect immunofluorescence test and Western blotting (WE). infected animals developed notable changes in serum protein levels reaching maximum protein concentrations 2-3 months postinfection (p.i.) related to the gamma-globulin fraction. Specific antibody titers were in good agreement with the serum protein rise, reaching immunofluorescence values of over 1:800 3 months p.i. Serial Western blotting analysis with L. infantum promastigotes protein showed a strong response against immunodominant antigens of 50-57, 42, and 29 kDa during most of the studied period with immunofluorescence titers of over 1:100 and in addition the response was remarkably homogeneous among the infected dogs. Immunoreactivity patterns displayed time-related variations; the response against 29 and 50-57 kDa was seen very early, followed by the reaction around 42, 76, and 86 kDa. In addition the recognition of peptides around 34-35.4 and 26 kDa was restricted to the acute phase of the experimental infection. Preliminary results obtained in naturally infected dogs seem to support the predictive value of the WB. (C) 1996 Academic Press, Inc.