A serine/threonine-specific protein kinase of Haemonchus contortus with a role in the development
A serine/threonine-specific protein kinase of Haemonchus contortus with a role in the development
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DOI：
10.1096/fj.201900888rr
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发表时间：
2019-12-27
期刊：
影响因子：
4.8
通讯作者：
Hu, Min
中科院分区：
文献类型：
--
作者：
Di, Wenda;Gasser, Robin B.;Hu, Min
在自由生活的线虫秀丽隐杆线虫中，丝氨酸/苏氨酸特异性蛋白激酶 AKT 已知通过胰岛素样信号通路在多尔形成、寿命和应激抵抗中发挥关键作用。尽管人们了解线虫 AKT 编码基因的结构和功能，但寄生线虫中的同源基因却并非如此。在本研究中，我们探索了寄生线虫捻转血矛线虫中的秀丽隐杆线虫 akt-1 基因同源物，研究了其转录亚型（Hc-akt-1a 和 Hc-akt-1b），并使用同源和异源功能基因组工具研究了表达和功能。在秀丽隐杆线虫中，我们发现 Hc-akt-1 的预测启动子驱动 N2（野生型）菌株的 ASJ 神经元中的大量表达。在 H. contortus（Haecon-5 染色）中，RNAi（浸泡）导致 Hc-akt-1a 和 Hc-akt-1b 的转录本丰度显着降低，并减少体外幼虫阶段幼虫的发育。化学抑制也被证明可以阻止幼虫发育。总而言之，这项研究的证据表明 Hc-akt-1 在 H. contortus 中发挥着关键的功能作用。
In the free-living nematode Caenorhabditis elegans, the serine/threonine-specific protein kinase, AKT, is known to play a key role in dauer formation, life-span, and stress-resistance through the insulin-like signaling pathway. Although the structure and function of AKT-coding genes of C. elegans are understood, this is not the case for homologous genes in parasitic nematodes. In the present study, we explored a C. elegans akt-1 gene homolog in the parasitic nematode Haemonchus contortus, investigated its transcript isoforms (Hc-akt-1a and Hc-akt-1b), and studied expression and function using both homologous and heterologous functional genomic tools. In C. elegans, we showed that the predicted promoter of Hc-akt-1 drives substantial expression in ASJ neurons of the N2 (wild-type) strain. In H. contortus (Haecon-5 stain), RNAi (soaking) led to a significantly decreased transcript abundance for both Hc-akt-1a and Hc-akt-1b, and reduced larval development in larval stages in vitro. Chemical inhibition was also shown to block larval development. Taken together, the evidence from this study points to a key functional role for Hc-akt-1 in H. contortus.