Stimulation of corticotropin-releasing hormone-mediated adrenocorticotropin secretion by central administration of prolactin-releasing peptide in rats
Stimulation of corticotropin-releasing hormone-mediated adrenocorticotropin secretion by central administration of prolactin-releasing peptide in rats
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DOI：
10.1016/s0304-3940(00)01077-6
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发表时间：
2000-05-19
影响因子：
2.5
通讯作者：
Fujino, M
中科院分区：
文献类型：
--
作者：
Matsumoto, H;Maruyama, M;Fujino, M
催乳素释放肽（Prolactin-releasing peptide，PrRP）是新近发现的一种下丘脑肽，是孤儿受体的内源性配体。我们以前证明，PrRP神经元广泛分布在整个大鼠脑，并建议PrRP可能有重要的功能，在中枢神经系统。为了分析PrRP的功能，我们研究了侧脑室注射（i. c. v.）PrRP给药对大鼠脑中c-Fos蛋白积聚的影响。结果清楚地表明，c-Fos蛋白的积累显着增加的促肾上腺皮质激素释放激素（CRH）阳性的小细胞神经分泌细胞在室旁核（PVN）的核。我们还证明了PrRP神经元和CRH细胞体之间的突触样接触，这表明PrRP 31对CRH分泌有一定的影响。因此，我们研究了静脉注射PrRP 31对CRH介导的促肾上腺皮质激素（ACTH）水平升高的影响，发现血浆ACTH水平确实因静脉注射PrRP 31而升高。此外，用静脉内α-螺旋CRH（一种有效的CRH拮抗剂）预处理的动物在i. c. v. PrRP 31给药后显示出减弱的血浆ACTH应答。这些结果强烈表明，PrRP影响下丘脑-垂体-肾上腺轴。(C)2000爱思唯尔科学爱尔兰有限公司保留所有权利。
Prolactin-releasing peptide (PrRP) is a recently isolated hypothalamic peptide which is an endogenous ligand to an orphan receptor. We previously demonstrated that PrRP neurons are widely distributed throughout the rat brain and suggested that PrRP may have important functions in the central nervous system. To analyze the function of PrRP, we studied the effect of intracerebroventricular (i.c.v.) PrRP administration on c-Fos protein accumulation in the rat brain. The results clearly indicated that c-Fos protein accumulation was dramatically increased in the nuclei of corticotropin-releasing hormone (CRH)-positive parvocellular neurosecretory cells in the paraventricular nucleus (PVN). We also demonstrated synapse-like contact between PrRP neurons and CRH cell bodies in the PVN, which suggests that PrRP31 has some effect on CRH secretion. We therefore investigated the effect of i.c.v, administration of PrRP31 on the CRH-mediated increase in adrenocorticotropin (ACTH) levels, and found that plasma ACTH levels were indeed increased by i.c.v. PrRP31. In addition, animals pre-treated with intravenous a-helical CRH, a potent CRH antagonist, showed attenuated plasma ACTH responses after i.c.v. PrRP31 administration. These results strongly suggest that PrRP affects the hypothalamic-pituitary-adrenal axis. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.