Mitochondrial genome of the Komodo dragon: Efficient sequencing method with reptile-oriented primers and novel gene rearrangements
Mitochondrial genome of the Komodo dragon: Efficient sequencing method with reptile-oriented primers and novel gene rearrangements
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DOI：
10.1093/dnares/11.2.115
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发表时间：
2004-04-30
期刊：
影响因子：
4.1
通讯作者：
Endo, H
中科院分区：
文献类型：
--
作者：
Kumazawa, Y;Endo, H
科莫多巨蜥（Varanus komodoensis）的线粒体基因组几乎完全测序，除了两个高度重复的非编码区。将长链聚合酶链反应（PCR）技术与爬行动物特异引物相结合，建立了一种适合于有鳞动物线粒体基因组测序的方法。结果发现，线粒体基因组具有新的基因排列，其中从NADH脱氢酶亚基6到脯氨酸tRNA的基因与重复的控制区域广泛地改组。这些对照区在700 bp以上具有99%的序列相似性。虽然蛇的线粒体基因组也被认为拥有几乎相同序列的重复控制区，但第二个控制区的位置表明，在导致蛇和科莫多龙的谱系上独立发生了重复。科莫多龙线粒体基因组的另一个特征是大量的串联重复序列，包括具有强二级结构的序列，这可能是复制中滑动链错配的位点。这些观察结果是一致的假设，串联重复通过滑动链错配可能会引起线粒体基因重排，并可能有助于保持类似的控制区的副本。
The mitochondrial genome of the Komodo dragon (Varanus komodoensis) was nearly completely sequenced, except for two highly repetitive noncoding regions. An efficient sequencing method for squamate mitochondrial genomes was established by combining the long polymerase chain reaction (PCR) technology and a set of reptile-oriented primers designed for nested PCR amplifications. It was found that the mitochondrial genome had novel gene arrangements in which genes from NADH dehydrogenase subunit 6 to proline tRNA were extensively shuffled with duplicate control regions. These control regions had 99% sequence similarity over 700 bp. Although snake mitochondrial genomes are also known to possess duplicate control regions with nearly identical sequences, the location of the second control region suggested independent occurrence of the duplication on lineages leading to snakes and the Komodo dragon. Another feature of the mitochondrial genome of the Komodo dragon was the considerable number of tandem repeats, including sequences with a strong secondary structure, as a possible site for the slipped-strand mispairing in replication. These observations are consistent with hypotheses that tandem duplications via the slipped-strand mispairing may induce mitochondrial gene rearrangements and may serve to maintain similar copies of the control region.