Integration of proteomic and genomic approaches to dissect seed germination vigor in Brassica napus seeds differing in oil content
Integration of proteomic and genomic approaches to dissect seed germination vigor in Brassica napus seeds differing in oil content
复制标题
整合蛋白质组学和基因组学方法来剖析不同含油量甘蓝型油菜种子的种子萌发活力
DOI：
10.1186/s12870-018-1624-7
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发表时间：
2019-01-11
影响因子：
5.3
通讯作者：
Li, Maoteng
中科院分区：
文献类型：
--
作者：
Gu, Jianwei;Hou, Dalin;Li, Maoteng
油菜（Brassica napus， B. napus）是世界上重要的油料作物。以往的研究表明，甘蓝型油菜种子萌发活力可能与种子含油量有关，但对种子萌发的调控机制尚不清楚。对种子萌发和萌发活力的调控机制进行剖析是必要的。结果采用蛋白质组学和基因组学方法分析了不同含油量甘蓝型油菜种子的萌发过程。采用二维荧光差异凝胶电泳（2D-DIGE）技术，对含油量高低的甘蓝型油菜萌发种子中165个差异表达蛋白（DEPs）进行了鉴定。蛋白质组学比较结果表明，含油量高的种子具有较高的代谢活性，尤其是硫氨基酸代谢活性。结果表明，高、低含油量种子在萌发过程中有31个独特基因发生了显著变化，其中13个基因位于萌发相关数量性状位点（qtl）的置信区间内，这些基因可能在调控种子萌发活力中起重要作用。结论本研究结果对了解甘蓝型油菜种子萌发活力的调控机制具有重要意义。
BackgroundRapeseed (Brassica napus, B. napus) is an important oil seed crop in the world. Previous studies showed that seed germination vigor might be correlated with seed oil content in B. napus, but the regulation mechanism for seed germination has not yet been explained clearly. Dissecting the regulation mechanism of seed germination and germination vigor is necessary.ResultsHere, proteomic and genomic approaches were used to analyze the germination process in B. napus seeds with different oil content. The identification of 165 differentially expressed proteins (DEPs) in the germinating seeds of B. napus with high and low oil content was accomplished by two-dimensional fluorescence difference in gel electrophoresis (2D-DIGE). The comparative proteomic results revealed that seeds with high oil content had higher metabolic activity, especially for sulfur amino acid metabolism. Thirty-one unique genes were shown to be significantly changed during germination between the seeds with high and low oil content, and thirteen of these genes were located within the confidence interval of germination-related quantitative trait locus (QTLs), which might play an important role in regulating seed germination vigor.ConclusionsThe present results are of importance for the understanding of the regulation mechanism for seed germination vigor in B. napus.