Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer
Comparison of visible and near-infrared wavelength-excitable fluorescent dyes for molecular imaging of cancer
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DOI：
10.1117/1.2717137
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发表时间：
2007-03-01
影响因子：
3.5
通讯作者：
Sevick-Muraca, Eva M.
中科院分区：
文献类型：
--
作者：
Adams, Kristen E.;Ke, Shi;Sevick-Muraca, Eva M.
靶向荧光分子成像探针可能提供检测疾病的最佳手段。稳定的有机荧光团可以在体内被传播光反复激发，因此可以为目标组织的图像检测提供大的信噪比（SNR）。在文献中，许多小动物成像研究是用与靶向组分缀合的红色可激发染料Cy5.5进行的。我们报告的近红外（NIR）和红色可激发染料的体内荧光成像性能的比较。将表皮生长因子（EGF）与Cy5.5 [激发/发射（ex/em），660/710 nm]或IRDye（R）800 CW（ex/em：785/830 nm）缀合，用于在皮下异种移植物模型中对EGF受体（EGFr）阳性（MDA-MB-468）和/或阴性（MDA-MB-435）人乳腺癌细胞系进行成像。静脉注射的共轭物在1纳摩尔染料当量与和没有抗EGFr单克隆抗体C225，预先管理24小时前作为竞争性配体EGFr。我们的图像显示，虽然两种药物都靶向EGFr，但EGF-IRDye（R）800 CW与EGF-Cy 5.5相比，背景显著降低，肿瘤与背景比（TBR）增加。免疫组织化学显示，EGF导致EGFr信号通路的激活，这表明在用作靶向诊断剂之前，应考虑潜在的有害作用。(C)2007年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。
Targeted fluorescent molecular imaging probes may provide an optimal means of detecting disease. Stable, organic fluorophores can be repeatedly excited in vivo by propagated light and consequentially can provide large signal-to-noise ratios (SNRs) for image detection of target tissues. In the literature, many small animal imaging studies are performed with a red excitable dye, Cy5.5, conjugated to the targeting component. We report the comparison of the in vivo fluorescent imaging performance of a near-IR (NIR) and a red-excitable dye. Epidermal growth factor (EGF) was conjugated with Cy5.5 [excitation/emission (ex/em), 660/710 nm] or IRDye (R) 800CW (ex/em: 785/830 nm) for imaging EGF receptor (EGFr) positive (MDA-MB-468) and/or negative (MDA-MB-435) human breast cancer cell lines in subcutaneous xenograft models. The conjugates were injected intravenously at 1-nmol-dye equivalent with and without anti-EGFr monoclonal antibody C225, preadministered 24 h prior as a competitive ligand to EGFr. Our images show that while both agents target EGFr, the EGF-IRDye (R) 800CW evidenced a significantly reduced background and enhanced the tumor-to-background ratio (TBR) compared to the EGF-Cy5.5. Immunohistochemistry shows that EGF causes activation of the EGFr signaling pathway, suggesting that prior to use as a targeting, diagnostic agent, potential deleterious effects should be considered. (C) 2007 Society of Photo-Optical Instrumentation Engineers.