MITOSIS-SPECIFIC MONOCLONAL-ANTIBODY MPM-2 INHIBITS XENOPUS OOCYTE MATURATION AND DEPLETES MATURATION-PROMOTING ACTIVITY
MITOSIS-SPECIFIC MONOCLONAL-ANTIBODY MPM-2 INHIBITS XENOPUS OOCYTE MATURATION AND DEPLETES MATURATION-PROMOTING ACTIVITY
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DOI：
10.1073/pnas.86.13.4982
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发表时间：
1989-07-01
影响因子：
11.1
通讯作者：
RAO, PN
中科院分区：
文献类型：
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作者：
KUANG, J;ZHAO, JY;RAO, PN
MPM-2是一种对有丝分裂中的细胞具有特异性的单克隆抗体，它识别一个具有共同磷酸化表位的蛋白质家族。在本研究中，我们已经表明，在非洲爪蟾卵母细胞成熟的孕酮诱导，磷酸化的MPM-2抗原与MPF活性的外观相一致。当在孕酮刺激之前将MPM-2（0.7-1.4 μ g/卵母细胞）注射到卵母细胞中时，MPF活性没有出现，并且成熟诱导被抑制，这通过生殖泡破裂和白点形成来判断。此外，MPM-2能够中和以及免疫耗竭MPF活性从有丝分裂的HeLa细胞和成熟的卵母细胞提取物。这些结果表明MPM-2识别MPF本身或调节MPF活性的蛋白，并且磷酸化MPM-2抗原的激酶可能是调节M期诱导的关键组分。
MPM-2, a monoclonal antibody specific for cells in mitosis, recognizes a family of proteins that share a common phosphorylated epitope. In this study we have shown that during the maturation of Xenopus laevis oocytes induced by progesterone, phoshorylation of MPM-2 antigens coincided with the appearance of MPF activity. When MPM-2 (0.7-1.4 .mu.g per oocyte) was injected into oocytes prior to progesterone stimulation, MPF activity failed to appear and induction of maturation was inhibited as judged by both germinal-vesicle breakdown and white-spot formation. Further, MPM-2 was able to neutralize as well as immunodeplete MPF activity from mitotic HeLa cell and mature oocyte extracts. These results suggest tht MPM-2 recognizes either MPF itself or a protein(s) that regulates MPF activity and that the kinase that phosphorylates MPM-2 antigens may be a key component in the regulation of M-phase induction.