Involvement of altered arginase activity, arginase I expression and NO production in accelerated intimal hyperplasia following cigarette smoke extract
Involvement of altered arginase activity, arginase I expression and NO production in accelerated intimal hyperplasia following cigarette smoke extract
复制标题
DOI：
10.1016/j.lfs.2008.07.016
复制
发表时间：
2008-09-26
期刊：
影响因子：
6.1
通讯作者：
Azuma, Hiroshi
中科院分区：
文献类型：
--
作者：
Nagai, Akiko;Imamura, Masatoshi;Azuma, Hiroshi
在目前的实验中，我们试图阐明是否参与了香烟烟雾提取物（CSE）给药后，内膜增生的加速，在血管生成酶活性的变化，蛋白表达的血管生成酶-I和-II，和NO的生产。通过球囊取栓导管去除雄性兔右颈动脉的内皮细胞引起内膜增生。左侧颈动脉行假手术，作为对照。通过将香烟烟雾流鼓泡到磷酸盐缓冲盐水中来制备CSE。从术前1周至术后4周，每天1次皮下注射CSE，连续5周。对样本进行组织学评估，并将内膜/中膜比率（%）作为内膜增生的指标进行评价。CSE加速内膜增生伴随着受损的环GMP产生的增加。增强了总的β-淀粉酶活性和β-淀粉酶-I的上调。Pearson相关系数分析表明，血管内皮细胞和平滑肌层的cAMP酶活性与内膜/中膜比值和cGMP的产生密切相关。这些结果表明，CSE增强的血管紧张素转换酶活性以及促进血管紧张素转换酶-I的上调，这与NO产生的增强损伤有关，为CSE后加速兔颈动脉内膜增生的相关过程提供了新的线索。皇冠版权所有（C）2008由爱思唯尔公司出版。All rights reserved.
In the present experiments, we tried to elucidate whether changes in arginase activity, protein expression of arginase-I and -II, and NO production are involved in accelerating the intimal hyperplasia following administration of cigarette smoke extract (CSE). The intimal hyperplasia was caused by removing endothelial cells with the aid of balloon embolectomy catheter in the right carotid artery of the male rabbit. The left carotid artery underwent sham operation and served as control. CSE was prepared by bubbling a stream of cigarette smoke into phosphate buffered saline. Rabbits were given subcutaneously with CSE once a day for 5 weeks from I week before to 4 weeks after the surgery. The specimens were assessed histologically and the intima/media ratio (%) was evaluated as an index of the intimal hyperplasia. The accelerated intimal hyperplasia with CSE was accompanied by the augmentation of the impaired cyclic GMP production. enhanced overall arginase activity and up-regulation of arginase-I. Pearson's correlation coefficient analyses revealed the close relationships among the arginase activities in endothelial cells and smooth muscle layer, the intimal/media ratio and cyclic GMP production. These results suggest that the enhanced arginase activity together with facilitated up-regulation of arginase-I with CSE, which was associated with the augmented impairment of NO production, shed a new light on the processes associated with accelerating the intimal hyperplasia in rabbit carotid arteries following CSE. Crown Copyright (C) 2008 Published by Elsevier Inc. All rights reserved.