Influence of gel properties on neocartilage formation by auricular chondrocytes photoencapsulated in hyaluronic acid networks
Influence of gel properties on neocartilage formation by auricular chondrocytes photoencapsulated in hyaluronic acid networks
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DOI：
10.1002/jbm.a.30660
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发表时间：
2006-06-01
影响因子：
4.9
通讯作者：
Burdick, Jason A.
中科院分区：
文献类型：
--
作者：
Chung, Cindy;Mesa, John;Burdick, Jason A.
本研究的目的是确定由于大分子单体分子量（50-1100 kDa）和大分子单体浓度（2-20 wt %）的变化，透明质酸（HA）水凝胶的网络结构和性质的变化如何影响由包封的耳廓软骨细胞形成的新软骨。为了研究组织形成，将猪耳廓软骨细胞光包封在各种网络中，皮下植入裸鼠背部，并在6周和12周后取出进行生化和组织学分析。12周后，各种构建体为81-93%的水，每个样品含有0.1 × 10（6）至0.6 × 10（6）个软骨细胞，并且由0-0.049 μ g硫酸软骨素/μ g湿重（糖胺聚糖（GAG）含量）和0.002-0.060 μ g胶原蛋白/μ g湿重组成。组织学染色显示，除了最小的I型胶原蛋白染色和强烈和均匀的11型胶原蛋白染色，在具有最大新软骨产生的结构中，软骨细胞和GAG的均匀分布。由2wt%的50 kDa HA大分子单体制造的水凝胶最类似于天然软骨的性质，并显示出最大的前景，用于软骨再生的持续发展。(c)2006 Wiley Periodicals，Inc.
The objective of this study was to determine how changes in the network structure and properties of hyaluronic acid (HA) hydrogels, due to variations in the macromer molecular weight (50-1100 kDa) and macromer concentration (2-20 wt %), affect neocartilage formation by encapsulated auricular chondrocytes. To investigate tissue formation, swine auricular chondrocytes were photoencapsulated in the various networks, implanted subcutaneously in the dorsum of nude mice, and explanted after 6 and 12 weeks for biochemical and histological analysis. After 12 weeks, the various constructs were 81-93% water, contained between 0.1 x 10(6) and 0.6 x 10(6) chondrocytes per sample, and consisted of 0-0.049 mu g chondroitin sulfate/mu g wet weight (glycosaminoglycan (GAG) content) and 0.002-0.060 mu g collagen/mu g wet weight. Histological staining showed an even distribution of chondrocytes and GAGs in addition to minimal type I collagen staining and intense and uniform type 11 collagen staining in the constructs with greatest neocartilage production. Hydrogels fabricated from 2 wt % of the 50 kDa HA macromer most resembled the properties of native cartilage and show the greatest promise for continued development for cartilage regeneration. (c) 2006 Wiley Periodicals, Inc.