Plant secondary metabolism engineering : methods and applications
Plant secondary metabolism engineering : methods and applications
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发表时间：
2010
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通讯作者：
A. Fett-Neto
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作者：
A. Fett-Neto
1.植物次生代谢和挑战，在修改其操作：概述奈拉戛纳做纳西曼托和亚瑟Germano Fett-Neto 2.利用抑制消减杂交技术鉴定苹果果皮花色素苷代谢相关基因Yusuke Ban和Takaya Moriguchi 3.使用瞬时RNAi系统鉴定参与生物碱生物合成的调节蛋白基因Yasuyuki Yamada，Nobuhiko Kato，Yasuhisa Kokabu，Qingyun Luo，Joseph Gogo Dubouzet，and Fumihiko Sato 4.定点突变和饱和突变用于植物次生代谢物生物合成中转录因子的功能研究。Werkman，Que Kong，and Ling Yuan 5.利用酵母单杂交Debora Vom Endt和Giancarlo Pasquali 6分离生物碱代谢相关基因启动子元件结合蛋白。通过增强库强度诱导质体形成调节转基因马铃薯中类胡萝卜素的积累Joyce货车Eck，Xiangjun Zhou，Shan Lu，and Li Li 7.过表达限速酶以提高生物碱生产率Tomoya Takemura，Yit-lai Chow，Takehiko Todokoro，Takuya Okamoto，and Fumihiko Sato 8.生物碱生物合成酶的微生物表达用于表征其性质Hiromichi Minami，Nobuhiro Ikezawa和Fumihiko Sato 9.在大肠杆菌中从小粒咖啡中生产重组黄素单加氧酶以筛选潜在的天然底物Igor Cesarino和Paulo Mazzafera 10。利用大肠杆菌在低温培养中高效生产活性形式的重组木薯羟基腈裂解酶Hisashi Semba，Eita Ichige，Tadayuki Imanaka，Haruyuki Atomi，and Hideki Aoyagi 11.利用SOE-PCR和同源杂交构建紫杉醇生物合成基因簇，在酵母中引入紫杉醇生物合成的早期途径12.生物催化合成氚（3 H）标记的紫杉-4（5），11（12）-二烯，紫杉烷二萜类化合物的前体Hans Schmeer和Stefan Jennewein 13.用户克隆和用户融合：小型和大型实验室的理想克隆技术。Nour-Eldin、Fernando Geu-Flores和Barbara A. 14. hallkier通过引入细菌八氢番茄红素合成酶基因Masaki Fujisawa和Norihiko Misawa 15富集亚麻籽中的类胡萝卜素。通过质体转化的代谢工程作为调节植物中类异戊二烯产量的策略Tomohisa Hasunuma，Akihiko Kondo和Chikahiro Miyake 16。通过用Rol基因转化在茜草细胞培养物中工程化高产量的次生代谢产物维克托P. Bulgakov，Yuri N. Shkryl和Galina N. 17. honeymoon流式细胞术方法研究植物代谢工程的培养异质性。Kolewe和Susan C. 18. baby baby罗勒叶和腺毛中苯丙素的生物合成西塞罗·德尚和詹姆斯·E.西蒙19.与荧光蛋白融合的植物生物碱生物合成酶的亚细胞定位帕特里夏杜阿尔特，约翰Memelink，和玛丽安娜索托马约尔20。葡萄浆果中一种推定的黄素转运蛋白的免疫组织化学定位Elisa Petrussa，Enrico Braidot，Marco Zancani，Carlo Peresson，Alberto Bertolini，Sonia Patui，Valentino Casolo，Sabina Passamonti，Francesco Macri，and Angelo Vianello 21.离体膜囊泡中的产电溴磺酞转运：
1. Plant Secondary Metabolism and Challenges in Modifying Its Operation: An Overview Naila Cannes do Nascimento and Arthur Germano Fett-Neto 2. Suppression Subtractive Hybridization as a Tool to Identify Anthocyanin Metabolism-Related Genes in Apple Skin Yusuke Ban and Takaya Moriguchi 3. Identification of Regulatory Protein Genes Involved in Alkaloid Biosynthesis Using a Transient RNAi System Yasuyuki Yamada, Nobuhiko Kato, Yasuhisa Kokabu, Qingyun Luo, Joseph Gogo Dubouzet, and Fumihiko Sato 4. Site-Directed Mutagenesis and Saturation Mutagenesis for the Functional Study of Transcription Factors Involved in Plant Secondary Metabolite Biosynthesis Sitakanta Pattanaik, Joshua R. Werkman, Que Kong, and Ling Yuan 5. Isolation of Proteins Binding to Promoter Elements of Alkaloid Metabolism-Related Genes Using Yeast One-Hybrid Debora Vom Endt and Giancarlo Pasquali 6. Modulation of Carotenoid Accumulation in Transgenic Potato by Inducing Chromoplast Formation with Enhanced Sink Strength Joyce Van Eck, Xiangjun Zhou, Shan Lu, and Li Li 7. Overexpression of Rate-Limiting Enzymes to Improve Alkaloid Productivity Tomoya Takemura, Yit-lai Chow, Takehiko Todokoro, Takuya Okamoto, and Fumihiko Sato 8. Microbial Expression of Alkaloid Biosynthetic Enzymes for Characterization of Their Properties Hiromichi Minami, Nobuhiro Ikezawa, and Fumihiko Sato 9. Producing a Recombinant Flavin-Containing Monooxygenase from Coffea arabica in Escherichia coli for Screening of Potential Natural Substrates Igor Cesarino and Paulo Mazzafera 10. Efficient Production of Active Form Recombinant Cassava Hydroxynitrile Lyase Using Escherichia coli in Low-Temperature-Culture Hisashi Semba, Eita Ichige, Tadayuki Imanaka, Haruyuki Atomi, and Hideki Aoyagi 11. Introduction of theEarly Pathway to Taxol Biosynthesis in Yeast by Means of Biosynthetic Gene Cluster Construction Using SOE-PCR and Homologous Recombination Pia Dahm and Stefan Jennewein 12. Biocatalytic Synthesis of Tritium (3H) Labelled Taxa-4(5),11(12)-diene, the Pathway Committing Precursor of the Taxoid Diterpenoids Hans Schmeer and Stefan Jennewein 13. USER Cloning and USER Fusion: The Ideal Cloning Techniques for Small and Big Laboratories Hussam H. Nour-Eldin, Fernando Geu-Flores, and Barbara A. Halkier 14. Enrichment of Carotenoids in Flaxseed by Introducing a Bacterial Phytoene Synthase Gene Masaki Fujisawa and Norihiko Misawa 15. Metabolic Engineering by Plastid Transformation as a Strategy to Modulate Isoprenoid Yield in Plants Tomohisa Hasunuma, Akihiko Kondo, and Chikahiro Miyake 16. Engineering High Yields of Secondary Metabolites in Rubia Cell Cultures through Transformation with Rol Genes Victor P. Bulgakov, Yuri N. Shkryl, and Galina N. Veremeichik 17. Flow Cytometric Methods to Investigate Culture Heterogeneities for Plant Metabolic Engineering Vishal Gaurav, Martin E. Kolewe, and Susan C. Roberts 18. Phenylpropanoid Biosynthesis in Leaves and Glandular Trichomes of Basil (Ocimum basilicum L.) Cicero Deschamps and James E. Simon 19. Fusion with Fluorescent Proteins for Subcellular Localization of Enzymes Involved in Plant Alkaloid Biosynthesis Patricia Duarte, Johan Memelink, and Mariana Sottomayor 20. Immunohistochemical Localization of a Putative Flavonoid Transporter in Grape Berries Elisa Petrussa, Enrico Braidot, Marco Zancani, Carlo Peresson, Alberto Bertolini, Sonia Patui, Valentino Casolo, Sabina Passamonti, Francesco Macri, and Angelo Vianello 21. Electrogenic Bromosulfalein Transport in Isolated Membrane Vesicles: