NEW HYPOTHESIS OF CONTACT GUIDANCE IN TISSUE-CELLS
NEW HYPOTHESIS OF CONTACT GUIDANCE IN TISSUE-CELLS
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DOI：
10.1016/0014-4827(76)90405-5
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发表时间：
1976-01-01
影响因子：
3.7
通讯作者：
HEATH, JP
中科院分区：
文献类型：
--
作者：
DUNN, GA;HEATH, JP
鸡心脏成纤维细胞对柱状基质的曲率的反应是使用基于沿和跨基质轴线的核尺寸的比率的测量来评估的。提出了接触引导假说，认为底物的形状对参与细胞运动的某些线状微丝束的形成施加了机械限制。这一假设被用来预测成纤维细胞在棱柱状基质上的行为，并得到了实验的证实。在80千伏和2毫伏条件下对鸡心脏成纤维细胞全铺展的透射电子显微镜观察发现，微丝束系统不连续，这与基质的形状不连续相吻合。
The reaction of chick heart fibroblasts to the curvature of cylindrical substrata was assessed using a measure based on the ratio of nuclear dimensions along and across the axis of the substratum. A hypothesis of contact guidance was proposed which states that the shape of the substratum imposes mechanical restrictions on the formation of certain linear bundles of microfilaments which are involved in cell locomotion. This hypothesis was used to predict fibroblast behavior on prism-shaped substrata and the prediction was confirmed by experiment. A transmission electron microscope examination of whole spread chick heart fibroblasts at 80 kV and 2 MV revealed discontinuities in the microfilament bundle system which coincided with a discontinuity in the shape of the substratum.