Isolation of the etiologic agent of human granulocytic ehrlichiosis from the white-footed mouse (Peromyscus leucopus).
Isolation of the etiologic agent of human granulocytic ehrlichiosis from the white-footed mouse (Peromyscus leucopus).
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从白足小鼠（Peromyscus leucopus）中分离出人粒细胞埃利希体病的病原体。
DOI：
10.1128/jcm.39.1.335-338.2001
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发表时间：
2001
影响因子：
9.4
通讯作者：
Johnson,RC
中科院分区：
文献类型：
--
作者：
Ravyn,MD;Kodner,CB;Carter,SE;Jarnefeld,JL;Johnson,RC
我们检查了白足小鼠（白足鼠）从明尼苏达州感染的病原体的人粒细胞埃立克体病（HGE）。从1997年4月到9月，我们收集了P。来自明尼苏达州华盛顿县HGE地方性流行区的白腹杆菌。在HL 60细胞中培养血液以分离HGE试剂。在检查的59只小鼠中，只有一只小鼠对HGE试剂培养呈阳性。分离物的16 S核糖体DNA序列被确定为与HGE试剂相同。该分离株与HGE试剂的44-kDa抗原的单克隆抗体反应，对实验室小鼠具有感染性。
We examined white-footed mice (Peromyscus leucopus) from Minnesota for infection with the etiologic agent of human granulocytic ehrlichiosis (HGE). From April to September 1997, we collectedP. leucopusfrom Washington County, Minnesota, an area enzootic for HGE. Blood was cultivated in HL60 cells for isolation of the HGE agent. Of 59 mice examined, only a single mouse was culture positive for the HGE agent. The 16S ribosomal DNA sequence of the isolate was determined to be identical to that of the HGE agent. The isolate was reactive with monoclonal antibodies to the 44-kDa antigen of the HGE agent and was infectious for laboratory mice.