Genetic analysis of a synaptic calcium channel in Drosophila: intragenic modifiers of a temperature-sensitive paralytic mutant of cacophony.
Genetic analysis of a synaptic calcium channel in Drosophila: intragenic modifiers of a temperature-sensitive paralytic mutant of cacophony.
复制标题
果蝇突触钙通道的遗传分析：温度敏感麻痹突变体的基因内修饰剂。
DOI：
10.1093/genetics/164.1.163
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发表时间：
2003
期刊：
影响因子：
3.3
通讯作者：
Ordway,RW
中科院分区：
文献类型：
--
作者：
Brooks,IM;Felling,R;Kawasaki,F;Ordway,RW
我们之前对果蝇突触机制的遗传分析发现了电压门控钙通道α1亚基基因cacophony（cac）的温度敏感麻痹突变体。对这个突变体cacts2的电生理研究表明，cacts2编码一个初级钙通道α1亚基，在神经递质释放中起作用。为了进一步研究cac编码钙通道的功能和相互作用，进行了遗传筛选，以分离修饰acts2麻痹表型的新突变。筛选发现了10个增强或抑制scacts2的突变，包括基因内修饰因子incac的第二位点突变以及指向其他基因的突变（基因外修饰因子）。在这里，我们报告了三种基因内修饰子的分子特征，并研究了这些突变对cacpre- mrna的温度敏感行为、突触功能和加工的影响。这些突变可能进一步确定钙通道α1亚基在神经递质释放中的功能结构基础。
Our previous genetic analysis of synaptic mechanisms in Drosophila identified a temperature-sensitive paralytic mutant of the voltage-gated calcium channel α1 subunit gene,cacophony(cac). Electrophysiological studies in this mutant, designatedcacTS2, indicatedcacencodes a primary calcium channel α1 subunit functioning in neurotransmitter release. To further examine the functions and interactions ofcac-encoded calcium channels, a genetic screen was performed to isolate new mutations that modify thecacTS2paralytic phenotype. The screen recovered 10 mutations that enhance or suppresscacTS2, including second-site mutations incac(intragenic modifiers) as well as mutations mapping to other genes (extragenic modifiers). Here we report molecular characterization of three intragenic modifiers and examine the consequences of these mutations for temperature-sensitive behavior, synaptic function, and processing ofcacpre-mRNAs. These mutations may further define the structural basis of calcium channel α1 subunit function in neurotransmitter release.