Phylogeography of a Specialist Leaf-Mining Weevil, Rhynchaenus dorsoplanatus (Coleoptera: Curculionidae), Associated With Castanopsis Species
Phylogeography of a Specialist Leaf-Mining Weevil, Rhynchaenus dorsoplanatus (Coleoptera: Curculionidae), Associated With Castanopsis Species
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DOI：
10.1603/an09157
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发表时间：
2010-05-01
影响因子：
2.3
通讯作者：
Kato, Makoto
中科院分区：
文献类型：
--
作者：
Aoki, Kyoko;Murakami, Noriaki;Kato, Makoto
冰川期的气候变化对生物体的近代进化史产生了重大影响，即使是在没有冰盖覆盖的暖温带森林中。我们调查了象甲，Rhynchaenus dorsoplanatus（Roelofs）（鞘翅目：天牛科），一个主机特定的叶矿工锥（壳斗科），生长在阔叶万年青林的日本。我们研究了2343 bp的线粒体DNA（mtDNA）的171个人收集新的侵染叶片在日本的55个地方。分子方差的空间分析表明，采叶红蜘蛛的遗传结构存在显著差异（Phi（CT）= 0.14，P < 0.001）。在日本主要岛屿内的西南和东北地区之间的背扁平鱼。我们还观察到西南部和东北部分支之间的巨大差距（Phi（CT），= 0.79，P < 0.001）相对于种子钻刺蛾的mtDNA序列hilgendoifi（Harold）（鞘翅目：刺蛾科），共享相同的寄主植物物种。人口的人口膨胀被检测到，并开始的时间膨胀的日期在两种象鼻虫的末次冰期结束。全等的地理格局中观察到的叶采矿和种子钻象鼻虫物种加强了稳健性推导的冰川和冰后期的历史Castanopsis相关的生物。这些数据表明，主机特定的寄生昆虫及其相关的锥树林可能仅限于单独的西南部和东北部避难所内的主要岛屿在第四纪重复冰期。
Climatic changes during glacial periods have had a major influence on the recent evolutionary history of living organisms, even in the warm temperate forests, where the land was not covered with ice sheets. We investigated the phylogeographical patterns of the weevil, Rhynchaenus dorsoplanatus (Roelofs) (Coleoptera: Curculionidae), a host-specific leaf miner of Castanopsis (Fagaceae) that grows in the broadleaved evergreen forests of Japan. We examined 2343 bp of mitochondrial DNA (mtDNA) for 171 individuals collected from new infested leaves of Castanopsis at 55 localities in Japan. Spatial analysis of molecular variance showed a significant difference (Phi(CT) = 0.14, P < 0.001) in genetic structure of the leaf-mining R. dorsoplanatus between the southwestern and the northeastern areas within the main islands of Japan. We also observed a large gap between the southwestern and northeastern clades (Phi(CT), = 0.79, P < 0.001) with respect to the mtDNA sequences of the seed-boring Curculio hilgendoifi (Harold) (Coleoptera: Curculionidae), which shares the same host plant species. The demographic population expansion was detected and the beginning time of the expansion was dated to the end of the last glacial period in both weevil species. The congruent phylogeographical patterns observed in the leaf-mining and seed-boring weevil species reinforce the robustness of the deduced glacial and postglacial history of Castanopsis-associated organisms. These data suggest that the host-specific parasitic insects and their associated Castanopsis forests were likely restricted to separate southwestern and northeastern refugia within the main islands of Japan during repeated glacial periods in the Quaternary.