Distribution of urocortin 3 neurons innervating the ventral premammillary nucleus in the rat brain
Distribution of urocortin 3 neurons innervating the ventral premammillary nucleus in the rat brain
复制标题
DOI：
10.1016/j.brainres.2006.03.043
复制
发表时间：
2006-05-17
期刊：
影响因子：
2.9
通讯作者：
Elias, Carol Fuzeti
中科院分区：
文献类型：
--
作者：
Cavalcante, Judney Cley;Sita, Luciane Valeria;Elias, Carol Fuzeti
尿皮质素3（Urocortin 3，UCN 3）是最近发现的一种促肾上腺皮质激素释放因子家族的多肽。表达Ucn 3 mRNA和肽的神经元分布在特定的脑区，包括正中视前核、穹窿周围区（PFx）和杏仁核内侧核（MEA）。对Ucn 3免疫反应的纤维局限于某些脑核团，在腹侧乳头体前核（PMV）中特别密集。在涉及电解损伤和Fos分布分析的研究中，根据行为范式，PMV可能与同种攻击和性行为有关。然而，UCN 3在这一通路中所起的作用尚未被探索。因此，我们研究了Wistar大鼠PMV的尿皮质素能神经支配的起源，试图绘制脑回路并确定可能的相关功能。我们将霍乱毒素B亚单位逆行注入PMV，发现PMV中88%的Ucn 3免疫反应纤维起源于背侧MEA，少数起源于PFx。作为对照，我们将顺行示踪剂生物素葡聚糖胺注射到这两个区域。我们观察到PMV受到MEA的密集神经支配，而PFx几乎没有神经支配。MEA是犁鼻系统的次级中继，并充分投射到与激素和行为调节相关的下丘脑核团，包括PMV。虽然生理学研究也应该进行，我们假设，UCN 3参与这样的途径，传递感觉信息的PMV，这反过来又调节行为和神经内分泌反应。(c)2006 Elsevier B.V.保留所有权利。
Urocortin 3 (Ucn 3) is a recently described peptide of the corticotropin-releasing factor family. Neurons expressing Ucn 3 mRNA and peptide are distributed in specific brain areas, including the median preoptic nucleus, the perifornical area (PFx), and the medial nucleus of the amygdala (MEA). Fibers immunoreactive to Ucn 3 are confined to certain brain nuclei, being particularly dense in the ventral premammillary nucleus (PMV). In studies involving electrolytic lesions and analysis of Fos distribution according to behavioral paradigms, the PMV has been potentially implicated in conspecific aggression and sexual behavior. However, the role that Ucn 3 plays in this pathway has not been explored. Therefore, we investigated the origins of the urocortinergic innervation of the PMV of Wistar rat in an attempt to map the brain circuitry and identify likely related functions. We injected the retrograde tracer cholera toxin b subunit into the PMV and found that 88% of the Ucn 3-immunoreactive fibers in the PMV originate in the dorsal MEA, and that few originate in the PFx. As a control, we injected the anterograde tracer biotin dextran amine into both regions. We observed that the PMV is densely innervated by the MEA, and scarcely innervated by the PFx. The MEA is a secondary relay of the vomeronasal system and projects amply to hypothalamic nuclei related to hormonal and behavioral adjustments, including the PMV. Although physiological studies should also be performed, we hypothesize that Ucn 3 participates in such pathways, conveying sensory information to the PMV, which in turn modulates behavioral and neuroendocrine responses. (c) 2006 Elsevier B.V. All rights reserved.