Human nectin3/PRR3: a novel member of the PVR/PRR/nectin family that interacts with afadin
Human nectin3/PRR3: a novel member of the PVR/PRR/nectin family that interacts with afadin
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DOI：
10.1016/s0378-1119(00)00316-4
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发表时间：
2000-09-19
期刊：
影响因子：
3.5
通讯作者：
Lopez, M
中科院分区：
文献类型：
--
作者：
Reymond, N;Borg, JP;Lopez, M
我们已经分离出nectin 3/PRR 3，nectin/PRR家族的第四个人类成员，也被描述为α疱疹病毒受体家族。Nectin/PRR成员是在细胞间连接处表达的粘附分子。Nectin 3/PRR 3是一种跨膜蛋白，其胞外区含有三个Ig样结构域（V、C和C），与其他成员的同源性约为30%。主要在睾丸和胎盘组织中表达。SDS-PAGE分析表明nectin 3/PRR 3的分子量为83 kDa。Nectin 1/PRR 1 L和Nectin 2/PRR 2S和L在细胞间连接处特异性表达。这种定位部分是由于这些受体的C-末端部分（由共有序列A/EXYV结束）和afadin的PDZ结构域的相互作用。在这份报告中，我们证明了nectin 3/PRR 3受体携带A/EXYV的共识序列，并在体内与长和短的afadin亚型相互作用。这些结果表明，人nectin 3/PRR 3是一个新的afadin相关分子。(C)2000 Elsevier Science B. V.保留所有权利。
We have isolated nectin3/PRR3, the fourth human member of the nectin/PRR family, also described as the alpha herpes virus receptor family. Nectin/PRR members are adhesion molecules expressed at intercellular junctions. Nectin3/PRR3 is a transmembrane protein, whose extracellular region contains three Ig-like domains (V, C and C) and shares approximately 30% identity with the other members. It is mainly expressed in testis and placental tissues. SDS-PAGE analyses demonstrate that nectin3/PRR3 has a molecular weight of 83 kDa. Nectin1/PRR1L and nectin2/PRR2S and L were found to be specifically expressed at the intercellular junctions. This localization is in part due to the interaction of the C-terminal part of these receptors (ended by the consensus sequence A/EXYV) and the PDZ domain of afadin. In this report we demonstrate that the nectin3/PRR3 receptor carries the A/EXYV consensus sequence and interacts in vivo with both long and short isoforms of afadin. These results suggest that the human nectin3/PRR3 is a new afadin-associated molecule. (C) 2000 Elsevier Science B.V. All rights reserved.