YEAST AND MAMMALS UTILIZE SIMILAR CYTOSOLIC COMPONENTS TO DRIVE PROTEIN-TRANSPORT THROUGH THE GOLGI-COMPLEX
YEAST AND MAMMALS UTILIZE SIMILAR CYTOSOLIC COMPONENTS TO DRIVE PROTEIN-TRANSPORT THROUGH THE GOLGI-COMPLEX
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DOI：
10.1073/pnas.83.6.1622
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发表时间：
1986-03-01
影响因子：
11.1
通讯作者：
ROTHMAN, JE
中科院分区：
文献类型：
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作者：
DUNPHY, WG;PFEFFER, SR;ROTHMAN, JE
哺乳动物高尔基体的连续隔室之间的水泡运输最近在无细胞系统中被重建。除了ATP外，转运还需要膜结合蛋白和细胞质蛋白。在这里，我们报告了酵母细胞质组分将有效地替代哺乳动物细胞质。哺乳动物细胞质溶胶含有几种不同的转运因子，我们已经在凝胶过滤和离子交换色谱的基础上进行了区分。酵母细胞质似乎含有相同的运输因子集合。来自酵母和哺乳动物的溶解细胞质因子以协同的方式相互补充。这些发现表明，细胞内蛋白质转运的分子机制在整个进化过程中一直是保守的。此外，这种杂交无细胞系统将使酵母遗传学应用于鉴定和分离维持细胞内蛋白质运输的胞质蛋白。
Vesicular transport between successive compartments of the mammalian Golgi apparatus has recently been reconstituted in a cell-free system. In addition to ATP, transport requires both membrane-bound and cytosolic proteins. Here we report that the cytosol fraction from yeast will efficiently substitute for mammalian cytosol. Mammalian cytosol contains several distinct transport factors, which we have distinguished on the basis of gel filtration and ion-exchange chromatography. Yeast cytosol appears to contain the same collection of transport factors. Resolved cytosol factors from yeast and mammals complement each other in a synergistic manner. These findings suggest that the molecular mechanisms of intracellular protein transport have been conserved throughout evolution. Moreover, this hybrid cell-free system will enable the application of yeast genetics to the identification and isolation of cytosolic proteins that sustain intracellular protein transport.