Expression of Various Types of Glycans in the Lugworm (Perinereis nuntia Var. Vallata)
Expression of Various Types of Glycans in the Lugworm (Perinereis nuntia Var. Vallata)
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发表时间：
2015
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通讯作者：
Y. Koide;Imtiaj Hasan;A. Asanuma;Yukio Fijii;Y. Ogawa;Hidemitsu Kobayashi;S. Rajia;S. Kawsar;R. Kanaly;Y. Ozeki
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作者：
Y. Koide;Imtiaj Hasan;A. Asanuma;Yukio Fijii;Y. Ogawa;Hidemitsu Kobayashi;S. Rajia;S. Kawsar;R. Kanaly;Y. Ozeki
海洋生物组织中的聚糖表达模式可以从不同角度提供有关糖生物学的有用信息。太平洋环节动物 Perinereis nuntia ver. 中存在寡糖结构的多样性。通过使用荧光标记的凝集素对 vallata（多毛类）进行了调查，以阐明其聚糖结合特性。组织化学分析显示环节动物中聚糖模式的多样性。除了整个组织外，还特别在肠壁周边发现了聚糖非还原末端的甘露糖。小麦胚芽凝集素检测到的聚糖与非还原性末端 N-乙酰氨基葡萄糖的反应在组织切片上较弱。相比之下，由 Ulex europaeus 凝集素-1 检测到的带有岩藻糖的聚糖仅存在于诺足的有限部分中。同时，Galβ1-3GalNAc/GlcNAc和β-半乳糖苷出现在身体周围的环肌处，并分别被同一区域的双孢蘑菇凝集素和刺桐凝集素检测到； NeuAcα2-6Gal/GalNAc 聚糖通过接骨木凝集素检测。然而，沙蚕凝集素在沙蚕组织中不存在NeuAcα2-3Gal/GalNAc。这些结果表明，β-半乳糖苷聚糖在多毛类的环形肌中表达，并存在部分唾液酸。通过凝集素缀合的琼脂糖凝胶从粗提物中分离环节动物中的糖蛋白。 P. nuntia 中表达的具有特征聚糖结构的糖缀合物的存在是一个新发现。
Glycan expression pattern in the tissue of marine organisms can provide useful information about glycobiology from different angles. The diversity of oligosaccharide structures present in the annelid from the Pacific Ocean, Perinereis nuntia ver. vallata (polychaeta), was surveyed by using fluorescent labelled lectins to elucidate their glycan-binding properties. Histochemical analyses showed the diversity of glycan patterns in the annelid. Mannose at the non-reducing terminus of glycans was found specifically at the periphery of the intestinal wall in addition to the whole tissue. The response of glycans with non-reducing terminal N-acetylglucosamine detected by Wheat germ agglutinin was weak on the tissue section. Contrastively, glycans with fucose detected by Ulex europaeus agglutinin-1 were present in limited parts of the notopodia. At the same time, Galβ1-3GalNAc/GlcNAc and β-galactoside appeared at the circular muscles surrounding the body and were detected by Agaricus bisporus agglutinin and Erythrina cristagalli agglutinin, respectively in the same region; NeuAcα2-6Gal/GalNAc glycan was detected by Sambucus sieboldiana agglutinin. However, NeuAcα2-3Gal/GalNAc was not present in the tissue of lugworm by Maackia amurensis agglutinin. These results indicated that β-galactoside glycans were expressed in the circular muscles of the polychaeta, with a partial presence of sialic acid. The glycoproteins in the annelid were isolated from the crude extract by lectinconjugated agarose gels. The presence of glycoconjugates expressed in P. nuntia with characteristic glycan structures is a novel finding.