ACID-PHOSPHATASE ACTIVITY AT NODES OF RANVIER IN ALPHA-MOTOR AND DORSAL-ROOT GANGLION NEURONS OF THE CAT
ACID-PHOSPHATASE ACTIVITY AT NODES OF RANVIER IN ALPHA-MOTOR AND DORSAL-ROOT GANGLION NEURONS OF THE CAT
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DOI：
10.1007/bf01189808
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发表时间：
1988-08-01
期刊：
影响因子：
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通讯作者：
CORNELIUSON, O
中科院分区：
文献类型：
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作者：
GATZINSKY, KP;BERTHOLD, CH;CORNELIUSON, O
本文用光镜和电镜对猫α-运动神经元和背根神经节（DRG）神经元的酸性磷酸酶（Acetylphosphatase，Acetylphosphatase）活性进行了组织化学分析。轴突内表达的Acceptor活性的发生和分布取决于神经元类型和与细胞体的距离。在α-运动和DRG神经元中，主要在Ranvier节点观察到各种形态类别的AcPase阳性小体，在那里它们更频繁地出现在节点中层的远端而不是近端。在这两种类型的神经元的外周定位的过程中，大多数较大的乙酰胆碱酯酶阳性机构与结旁轴突雪旺细胞网络。在位于中央的突起中，ACP酶阳性小体位于收缩的轴突段和邻近的结旁轴浆中。在运动和DRG轴突中，AcPase阳性小体在脊神经根水平比在PNS-CNS边界中央水平更频繁。观察结果表明，溶酶体（即AcPase-positive机构）构成轴突内系统的细胞器在正常的，大的，有髓鞘的α-电机和DRG的猫轴突的一部分。溶酶体介导的降解逆行运输的内源性和外源性材料可能是广泛的在正常的外周定向神经元过程。这项研究还表明，PNS和CNS部分相同的轴突之间的差异，关于溶酶体细胞器的局部营业额。
Acid phosphatase (AcPase) activity in feline alpha-motor and dorsal root ganglion (DRG) neurons was analysed histochemically by light and electron microscopy. The occurrence and distribution of the AcPase activity expressed within the axon differed depending on neuron type and distance from the cell body. Both in alpha-motor and DRG neurons, AcPase-positive bodies of various morphological categories were observed mainly at nodes of Ranvier, where they were more frequent distal than proximal to the nodal midlevel. In the peripherally located processes of both neuron types, most of the larger AcPase-positive bodies were associated with the paranodal axon-Schwann cell network. In the centrally located processes the AcPase-positive bodies were situated in the constricted axon segment and the adjacent paranodal axoplasm. Both in motor and DRG axons, AcPase-positive bodies were more frequent at the spinal root level than at a level central to the PNS-CNS borderline. The observations indicate that lysosomes (i.e. AcPase-positive bodies) constitute part of the intra-axonal system of organelles in normal, large, myelinated alpha-motor and DRG axons of the cat. Lysosome-mediated degradation of retrogradely transported endogenous andexogenous materials may be extensive in normal peripherally directed neuronal processes. The study also suggests a difference between PNS and CNS parts of the same axon with regard to the local turnover of lysosomal organelles.