A possible stimulus to induce SPAWNING in Ezo abalone Haliotis discus hannai during stormy weather: Fenton reaction induces spawning behavior
A possible stimulus to induce SPAWNING in Ezo abalone Haliotis discus hannai during stormy weather: Fenton reaction induces spawning behavior
复制标题
暴风雨天气期间诱导虾夷鲍鱼产卵的可能刺激：芬顿反应诱导产卵行为
DOI：
10.1101/2020.10.30.356469
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Yatsuya Kousuke
中科院分区：
文献类型：
--
作者：
Matsumoto Yukio;Yatsuya Kousuke
同步产卵是无脊椎动物体外受精提高受精成功率的重要行为。以前的工作表明，在水槽实验中使用自由基诱导产卵行为是可能的，但在野外产卵的刺激还没有完全理解。皱纹盘鲍主要在暴风雨天气产卵。雨水中含有H2 O2和铁（II）离子（Fe 2+）。我们建议，在暴风雨天气在海洋中的水层是混合的，含有H2 O2和Fe 2+的表面层与海底相互作用，这导致了适合芬顿反应发生的条件。H_2O_2氧化Fe ~（2+）时产生的羟基自由基（·OH）可能诱导野生鲍的产卵行为。本研究观察到H.在暴风雨条件下，由于降雨导致盐度降低，皱纹盘鱼产卵。此外，我们的水槽实验表明，由芬顿反应产生的·OH诱导两性之间的同步产卵行为。本研究为控制黑鲷的同步产卵提供了一个新的假说。本文的研究结果也适用于其它无脊椎动物。
Synchronous spawning is an important behavior to increase fertilization success of invertebrates with external fertilization. Previous work has shown that it is possible to induce spawning behavior using free radicals in tank experiments, but the stimulus for spawning in the wild is not fully understood. Ezo abaloneHaliotis discus hannaimainly spawn during stormy weather. Rainwater contains H2O2and iron (II) ions (Fe2+). We propose that during stormy weather water layers in the ocean are mixed and the surface layer containing H2O2and Fe2+interacts with the ocean bottom; this leads to conditions suitable for the Fenton reaction to occur. Hydroxyl radicals (·OH) are generated during the oxidization of Fe2+by H2O2and we hypothesized these induce spawning behavior of abalone in the wild. This study observed thatH. discus hannaireleased eggs after salinity decreased due to the rainfall during stormy conditions. In addition, our tank experiment demonstrated that ·OH generated by the Fenton reaction induced synchronous spawning behavior between the sexes. This study provides a new hypothesis about control of synchronous spawning inH. discus hannaiand the results could be applicable to other invertebrates.