Evidence for thyroid hormone-dependent and independent glucocorticoid actions in cultured cells. Studies on the induction of growth hormone and glutamine synthetase in GH1 cells and tyrosine aminotransferase in Reuber H-35 cells.
Evidence for thyroid hormone-dependent and independent glucocorticoid actions in cultured cells. Studies on the induction of growth hormone and glutamine synthetase in GH1 cells and tyrosine aminotransferase in Reuber H-35 cells.
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培养细胞中甲状腺激素依赖性和独立糖皮质激素作用的证据。
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
J. Casanova
中科院分区：
文献类型：
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作者：
H. Samuels;D. Klein;F. Stanley;J. Casanova
在培养的GH1细胞中，甲状腺激素对生长激素合成和mRNA的诱导是由甲状腺激素核受体介导的。此外，糖皮质激素对生长激素反应的调节高度依赖于甲状腺激素的作用。为了阐明甲状腺激素是否对GH1细胞中的糖皮质激素作用具有普遍影响，同时检查了生长激素和谷氨酰胺合成酶的糖皮质激素诱导。与生长激素反应相反，糖皮质激素对谷氨酰胺合成酶的诱导不受甲状腺激素的影响。这两种反应似乎都是由糖皮质激素受体调节的，甲状腺激素对核相关糖皮质激素受体水平没有影响。这些结果表明，甲状腺激素控制糖皮质激素诱导生长激素可能是一个选择性的过程，甲状腺和糖皮质激素的核相关受体相互关联，以控制生长激素的反应。
The induction of growth hormone synthesis and mRNA by thyroid hormone in cultured GH1 cells is mediated by the thyroid hormone nuclear receptor. In addition, the regulation of the growth hormone response by glucocorticoid is highly dependent on the action of thyroid hormone. To clarify whether thyroid hormone has a general influence on glucocorticoid action in GH1 cells, the glucocorticoid induction of growth hormone and glutamine synthetase was simultaneously examined. In contrast to the growth hormone response, the induction of glutamine synthetase by glucocorticoid was not influenced by thyroid hormone. Both responses appear to be modulated by the glucocorticoid receptor, and thyroid hormone had no influence on nuclear-associated glucocorticoid receptor levels. These results suggest that the thyroid hormone control of glucocorticoid induction of growth hormone may be a selective process, and the nuclear associated receptors for both thyroid and glucocorticoid hormones interrelate to control the growth hormone response.