Comparison of blood plasma sample preparation methods for combined LC-MS lipidomics and metabolomics.
Comparison of blood plasma sample preparation methods for combined LC-MS lipidomics and metabolomics.
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DOI：
10.1016/j.jchromb.2015.08.018
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发表时间：
2015-10-01
期刊：
影响因子：
--
通讯作者：
Yost RA
中科院分区：
文献类型：
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作者：
Patterson RE;Ducrocq AJ;McDougall DJ;Garrett TJ;Yost RA
本研究的目的是找到最全面的血浆脂质提取方法，同时也为代谢物分析提供足够的水溶液制剂。之前已经对Folch、Bligh-Dyer和Matyash脂质提取进行了比较;此外，本文提供了用于分析的磷脂去除板的额外比较。该平板用于脂质提取，而不是其预期用于极性分析的脂质去除，并且证明其对于靶向脂质分析具有耐用性。Folch和Matyash在一系列脂质类别中提供了可重现的回收率，但Matyash水层与用于极性代谢物分析的典型甲醇制剂相比效果良好。因此，Matyash方法是血浆中代谢组学和脂质组学非靶向双相提取的最佳选择。
The goal of this research was to find the most comprehensive lipid extraction of blood plasma, while also providing adequate aqueous preparation for metabolite analysis. Comparisons have been made previously of the Folch, Bligh-Dyer, and Matyash lipid extractions; furthermore, this paper provides an additional comparison of a phospholipid removal plate for analysis. This plate was used for lipid extraction rather than its intended use in lipid removal for polar analysis, and it proves to be robust for targeted lipid analysis. Folch and Matyash provided reproducible recovery over a range of lipid classes, however the Matyash aqueous layer compared well to a typical methanol preparation for polar metabolite analysis. Thus, the Matyash method is the best choice for an untargeted biphasic extraction for metabolomics and lipidomics in blood plasma.